Volume 5, Issue 19 (7-2015)
NCMBJ 2015, 5(19): 85-94 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
shirmardi S P, Erfani M, zare A, Sarzaeem A. Preparation and investigation of ICD-85 biodistribution by 131I. NCMBJ 2015; 5 (19) :85-94
URL: http://ncmbjpiau.ir/article-1-654-en.html
URL: http://ncmbjpiau.ir/article-1-654-en.html
Radiation Application Research School, Nuclear Science and Technology Research Institute , mgandomkar@aeoi.org.ir
Abstract: (8341 Views)
Aims and Background: ICD-85 with weight of 10,000 to 30,000 Daltons is a triple peptide derived from Iranian brown snake venom, scorpion venom of Agkistrodon Halys and yellow Hemiscorpius lepturus. This compound inhibits cancer cells by inducing apoptosis. In this study, the preparation and labeling of this triple peptide has been done.
Materials and Methods: At first, these triple peptides were isolated from snake and yellow scorpion venom by gel and high performance liquid chromatography. Labeling with iodine-131 was performed through chloramine-T method and radiochemical analysis involved sephadex G-25 method. The stability and biodistribution of radio peptide was checked in the presence of PBS and mice respectively.
Results: The results of biodistribution showed that the labeled compound has about 15% accumulation in breast tumor and in other organs such as liver and kidney they have the highest radio peptide concentration. Tumor to blood ratio for radio peptide is more than 293 percent. Conclusion: The labeled ICD-85 by 131I can be an agent for diagnosis of breast cancer tumors.
Materials and Methods: At first, these triple peptides were isolated from snake and yellow scorpion venom by gel and high performance liquid chromatography. Labeling with iodine-131 was performed through chloramine-T method and radiochemical analysis involved sephadex G-25 method. The stability and biodistribution of radio peptide was checked in the presence of PBS and mice respectively.
Results: The results of biodistribution showed that the labeled compound has about 15% accumulation in breast tumor and in other organs such as liver and kidney they have the highest radio peptide concentration. Tumor to blood ratio for radio peptide is more than 293 percent. Conclusion: The labeled ICD-85 by 131I can be an agent for diagnosis of breast cancer tumors.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2015/07/16 | Accepted: 2015/07/16 | Published: 2015/07/16
Received: 2015/07/16 | Accepted: 2015/07/16 | Published: 2015/07/16
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
