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:: Volume 8, Issue 29 (1-2018) ::
NCMBJ 2018, 8(29): 97-106 Back to browse issues page
Isolation of bla_vim gene in Antibiotic resistant Pseudomonas aeruginosa from hospitals
Elham Siasi , Robab Rafiei Tabatabaii , Fatemeh MoslehiMehr
Department of Microbiology, School of science, Islamic Azad University, North Tehran Branch, Tehran, Iran , emi_biotech2006@yahoo.ca
Abstract:   (772 Views)
Aim and Background: Overplay of antibiotics in clinical centers not only did not destroy pathogenic bacterias but also grew a resistance for these kind of compounds in microorganisms such as Pseudomonas aeruginosa that is one of important infection factors in hospitals. The purpose of this study is to investigate the resistant gene to antibiotic, bla_vim gene, which was found among Pseudomonas aeruginosas released from hospitals.
Materials and Methods: 120 strains of Pseudomonas aeruginosas were collected from hospitals. The sensitivity of these strains to antibiotics was determined by Disc diffusion test. PCR was used for identification of bla_vim gene .
Results: Among 120 samples isolated from hospitals 50 cases were Pseudomonas aeruginosa. 35 Pseudomonas aeruginosa samples were resistant to antibiotics. 21 samples (42%) of 35 samples contained bla_vim gene.
Conclusion: The results of this study indicated the presence of bla _vim gene in Pseudomonas aeruginosa. The comparison of the results with the rest of the world indicates the increasing genetic resistance of Pseudomonas aeruginosa to antibiotics especially beta-lactams. These results are important in taking proper health policies and using effective antibiotics for hospital infections.
Keywords: Pseudomonas aeruginosa, antibiotic resistance, bla _vim gene, beta-lactams
Full-Text [PDF 577 kb]   (206 Downloads)    
Type of Study: Research Article | Subject: Microbiology
Received: 2018/02/28 | Accepted: 2018/02/28 | Published: 2018/02/28
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Siasi E, Rafiei Tabatabaii R, MoslehiMehr F. Isolation of bla_vim gene in Antibiotic resistant Pseudomonas aeruginosa from hospitals. NCMBJ. 2018; 8 (29) :97-106
URL: http://ncmbjpiau.ir/article-1-1063-en.html

Volume 8, Issue 29 (1-2018) Back to browse issues page
مجله تازه های بیوتکنولوژی سلولی - مولکولی New Cellular and Molecular Biotechnology Journal
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