Volume 10, Issue 39 (6-2020)                   NCMBJ 2020, 10(39): 61-78 | Back to browse issues page

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Hekmat A, Mohsenpour K, Atyabi S M, Bakhshandeh H. The Effects of Titanium dioxide Nanoparticles Coatings in the extent of their Effectiveness: DNA Interaction. NCMBJ. 2020; 10 (39) :61-78
URL: http://ncmbjpiau.ir/article-1-1299-en.html
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract:   (245 Views)
Aim and background: Among semiconductor nanoparticles, the titanium dioxide nanoparticles (TiO2NPs) are being widely consumed in the nanotechnology industry and medicine. TiO2NPs are synthesized in various ways. The purpose of this study was to investigate the effect of coating in the synthesis of TiO2NPs on their ability to induce structural disorder in DNA.
Materials and methods: Ultraviolet spectroscopy, fluorescence emission spectroscopy, circular dichroism (CD) spectroscopy, and zeta potential analysis were used to study the structural effects of DNA after interaction with TiO2NPs with ethylene glycol dispersion matrix, TiO2NPs contains HNO3 stabilizer and uncoated TiO2NPs.
Results: The Ultraviolet spectroscopy, fluorescence quenching and binding constants indicated that TiO2NPs with ethylene glycol dispersion matrix interacted with TiO2NPs with a higher binding constant value (2.2Í105 M-1) compare with two other TiO2NPs (2.3Í104 M-1 for uncoated TiO2NPs and 1.1Í105 M-1 for TiO2NPs contains HNO3 stabilizer). The CD spectra indicated TiO2NPs have significant influence on the DNA base stacking. The zeta potential analysis showed the surface charge of DNA especially after the addition of TiO2NPs with ethylene glycol dispersion matrix (-24.60 mV) was reduced.
Conclusion: This study showed that TiO2NPs with ethylene glycol dispersion matrix interact with DNA more strongly compared with two other TiO2NPs. This study indicated that the type of coating of TiO2NPs affects the DNA interaction and consequently the type of coating of nanoparticles is very important in designing nano-drugs especially for cancer treatment.
Full-Text [PDF 1453 kb]   (18 Downloads)    
Type of Study: Research Article | Subject: Cellular and molecular
Received: 2020/08/15 | Accepted: 2020/06/30 | Published: 2020/06/30

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