Volume 15, Issue 58 (4-2025)
NCMBJ 2025, 15(58): 0-0 |
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Sadeghloo Z, Safarian P, Hakemivala M, Sadeghi A, yadegar A. Investigating the effect of probiotic strain Lactobacillus gasseri on induction of apoptosis in AGS cells treated with extracellular vesicles of Helicobacter pylori. NCMBJ 2025; 15 (58)
URL: http://ncmbjpiau.ir/article-1-1737-en.html
URL: http://ncmbjpiau.ir/article-1-1737-en.html
Food and Waterborne Diseases Research Center, Institute of Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran , babak_y1983@yahoo.com
Abstract: (154 Views)
Aime and Background: Helicobacter pylori infection is known as the main risk factor associated with chronic gastritis and gastric adenocarcinoma. Extracellular vesicles are among the key virulence factors of this bacterium, which play a role in inducing inflammation and inhibiting apoptosis process in host cells. Probiotic strains, including Lactobacillus species, can play a role in inducing the process of cellular apoptosis. This study aimed to evaluate the effect of L gasseri ATCC 33323 on the apoptosis process in AGS cells treated with extracellular vesicles of H. pylori.
Materials and Methods: EVs were isolated from two clinical strains of H. pylori (BY-1 and OC824). The morphology of EVs was identified by transmission electron microscopy and dynamic light scattering. The viability of AGS cells was examined in the presence of various amounts of H. pylori EVs and L. gasseri cell-free supernatant (CFS). Based on MTT assay, 50 μg/ml of H. pylori EVs and 10% v/v of L. gasseri CFS were used for further experiments. Apoptosis was investigated using RT-qPCR targeting key apoptosis mediators (bax, cas3, cas8, cas9, bcl-2, and nod1).
Results: H. pylori EVs were identified in the size range of 50-200 nm. Based on the results of cell viability assays in AGS cells, 50 micrograms per milliliter of Helicobacter pylori extracellular vesicles and 10% (v/v) of L. gasseri culture supernatant were used for cell treatment. H. pylori EVs downregulated the gene expression of bax, cas3, cas8, and cas9 and upregulated bcl-2 and nod1 in AGS cells at 12 and 24 h. In contrast, L. gasseri CFS effectively induced cell apoptosis which was inhibited by EVs.
Conclusion: The present study showed that L. gasseri CFS can effectively induce H. pylori EV-inhibited apoptosis in AGS cells. Further research is needed to elucidate the precise function of L. gasseri CFS and its metabolites in induction of apoptosis.
Materials and Methods: EVs were isolated from two clinical strains of H. pylori (BY-1 and OC824). The morphology of EVs was identified by transmission electron microscopy and dynamic light scattering. The viability of AGS cells was examined in the presence of various amounts of H. pylori EVs and L. gasseri cell-free supernatant (CFS). Based on MTT assay, 50 μg/ml of H. pylori EVs and 10% v/v of L. gasseri CFS were used for further experiments. Apoptosis was investigated using RT-qPCR targeting key apoptosis mediators (bax, cas3, cas8, cas9, bcl-2, and nod1).
Results: H. pylori EVs were identified in the size range of 50-200 nm. Based on the results of cell viability assays in AGS cells, 50 micrograms per milliliter of Helicobacter pylori extracellular vesicles and 10% (v/v) of L. gasseri culture supernatant were used for cell treatment. H. pylori EVs downregulated the gene expression of bax, cas3, cas8, and cas9 and upregulated bcl-2 and nod1 in AGS cells at 12 and 24 h. In contrast, L. gasseri CFS effectively induced cell apoptosis which was inhibited by EVs.
Conclusion: The present study showed that L. gasseri CFS can effectively induce H. pylori EV-inhibited apoptosis in AGS cells. Further research is needed to elucidate the precise function of L. gasseri CFS and its metabolites in induction of apoptosis.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2025/04/27 | Accepted: 2025/04/3 | Published: 2025/04/3
Received: 2025/04/27 | Accepted: 2025/04/3 | Published: 2025/04/3
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