Volume 16, Issue 62 (5-2026)
NCMBJ 2026, 16(62): 49-62 |
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Hemmati Dezaki Z, Parivar K, Goodarzi V, Nourani M R. Fabrication and Evaluation of a Cobalt-Containing Three-Layer Nanofibrous Scaffold and Its Antibacterial Properties. NCMBJ 2026; 16 (62) :49-62
URL: http://ncmbjpiau.ir/article-1-1821-en.html
URL: http://ncmbjpiau.ir/article-1-1821-en.html
Tissue Engineering and Regenerative Medicine Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract: (19 Views)
Background and Aim: Bioactive glass has emerged as a novel innovation in materials science, offering extensive potential in biomedical applications. Due to its unique physicochemical and biological properties, it has become a prominent candidate in regenerative medicine. One of the remarkable features of bioactive glass is its intrinsic antimicrobial activity. In this study, cobalt ions were incorporated into a three-layer electrospun scaffold to enhance its antibacterial performance.
Materials and Methods: The scaffold was fabricated using a combination of polyurethane, polycaprolactone, and collagen, blended with cobalt-doped bioactive glass. The scaffold was produced in a three-layered structure through electrospinning. Its structural and biological properties were analyzed using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and cell adhesion assays. Antibacterial assessments were conducted using four bacterial strains, including both Gram-positive and Gram-negative species.
Results: The antibacterial evaluation conducted 24 hours post-bacterial seeding revealed that the fabricated scaffold effectively inhibited bacterial growth around the scaffold area. The results confirmed the scaffold's favorable antibacterial performance.
Conclusion: The findings of this study demonstrated that the incorporation of cobalt-containing bioactive glass into the designed scaffold significantly enhanced fibroblast cell proliferation and viability, while also exerting strong antibacterial effects. Therefore, this scaffold holds great potential as a promising candidate for tissue engineering applications in the repair of damaged tissues.
Materials and Methods: The scaffold was fabricated using a combination of polyurethane, polycaprolactone, and collagen, blended with cobalt-doped bioactive glass. The scaffold was produced in a three-layered structure through electrospinning. Its structural and biological properties were analyzed using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and cell adhesion assays. Antibacterial assessments were conducted using four bacterial strains, including both Gram-positive and Gram-negative species.
Results: The antibacterial evaluation conducted 24 hours post-bacterial seeding revealed that the fabricated scaffold effectively inhibited bacterial growth around the scaffold area. The results confirmed the scaffold's favorable antibacterial performance.
Conclusion: The findings of this study demonstrated that the incorporation of cobalt-containing bioactive glass into the designed scaffold significantly enhanced fibroblast cell proliferation and viability, while also exerting strong antibacterial effects. Therefore, this scaffold holds great potential as a promising candidate for tissue engineering applications in the repair of damaged tissues.
Type of Study: Research Article |
Subject:
Microbiology
Received: 2026/06/20 | Accepted: 2026/05/31 | Published: 2026/05/31
Received: 2026/06/20 | Accepted: 2026/05/31 | Published: 2026/05/31
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