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Identification of Mycoplasma gallisepticum in rural domestic poultry of Urmia city in the year 1395
Nasrin Hosseini , Mahdi Dilmaghani
Department of Cellular and Molecular biology; WAVL, Urmia, Iran , Mdilmaghani@gmail.com
Abstract:   (203 Views)
Aim and[PARANDCO1]  Bachground:
Mycoplasma gallisepticum is the most economically significant mycoplasma pathogen of poultry, and has a world-wide distribution. Infection with M. gallisepticum has a wide variety of clinical manifestations, but even in the absence of overt clinical signs, the economic impact may be significant.
Materials and methods:
Blood and swab samples were collected from 145 birds in sterile condition and transferred to lab with ice. Rapid Agglutination test was performed on blood samples and PCR assay targeting 16S rRNA was done on swab samples following DNA extraction.   
Results:
Based on Rapid Serum Agglutination Test on 145 samples, 24/8% of rural domestic poultry were positive and PCR using 16S rRNA gene primers denoted that 63/4%of samples are positive.
Conclusion:
Reliable methods for the discrimination of MG strains play an essential role in understanding the epidemiology and spread of the disease because they generate the information required for recognising and following new outbreaks. Genotyping methods also have a high degree of reproducibility, which is particularly important for the construction of reliable databases. Furthermore, genotyping methods are easy to interpret and can be performed rapidly.

 [PARANDCO1]براساس خلاصه فارسی اصلاح گردد
Keywords: Mycoplasma gallisepticum, Rapid Serum Agglutination, Rural domestic poultry, PCR, 16S rRNA gene.
     
Type of Study: Research Article | Subject: Cellular and molecular
Received: 2018/10/14 | Accepted: 2018/10/14 | Published: 2018/10/14
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مجله تازه های بیوتکنولوژی سلولی - مولکولی New Cellular and Molecular Biotechnology Journal
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