Volume 15, Issue 59 (7-2025)
NCMBJ 2025, 15(59): 19-34 |
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Siasi E, Aleyasin A, Mowla J. Association of single nucleotide polymorphisms T824C and T132903C in OR2W3 and INSR genes, respectively with idiopathic infertility in Iranian men. NCMBJ 2025; 15 (59) :19-34
URL: http://ncmbjpiau.ir/article-1-1766-en.html
URL: http://ncmbjpiau.ir/article-1-1766-en.html
. Associate Professor, Department of Genetic, School of science, NT.C., Islamic Azad University, Tehran, Iran. , emi_biotech2006@yahoo.ca
Abstract: (173 Views)
Aim and Background: The Frequency of male infertility is 10-15% and there is no definite reason in 50% of infertility in men. This form of infertility is named idiopathic infertility, and genetic factors are one of the causes of male infertility. Therefore, this study aimed to investigate on association between the T824C polymorphism in the OR2W3 gene and the T132903C polymorphism in the INSR gene in Iranian idiopathic infertile males.
Materials and Methods: For this study, DNA was extracted from 200 blood samples, consisting of 100 men with oligospermia and azoospermia (idiopathic infertile) and 100 fertile men as a control group. Genotyping of the two studied polymorphisms was performed by using molecular methods, HRM-PCR, PCR-RFLP, and sequencing, and finally, the results were statistically analyzed.
Results: For the T824C polymorphism in the OR2W3 gene, statistical analysis showed no significant difference between the patient and control groups (p = 0.2), and the results showed no association between this polymorphism and idiopathic male infertility in the Iranian population. In addition, the frequency of T132903C polymorphism in the INSR gene was different in infertile patients and control groups (p = 0.02) and indicates a significant relationship with idiopathic male infertility in the studied Iranian men population.
Conclusion: According to these research results, the T132903C polymorphism in the INSR gene is affected by idiopathic infertility in the Iranian male population and could cause oligospermia and azoospermia in Iranian infertile males. To confirm these results, it is recommended to study more samples.
Materials and Methods: For this study, DNA was extracted from 200 blood samples, consisting of 100 men with oligospermia and azoospermia (idiopathic infertile) and 100 fertile men as a control group. Genotyping of the two studied polymorphisms was performed by using molecular methods, HRM-PCR, PCR-RFLP, and sequencing, and finally, the results were statistically analyzed.
Results: For the T824C polymorphism in the OR2W3 gene, statistical analysis showed no significant difference between the patient and control groups (p = 0.2), and the results showed no association between this polymorphism and idiopathic male infertility in the Iranian population. In addition, the frequency of T132903C polymorphism in the INSR gene was different in infertile patients and control groups (p = 0.02) and indicates a significant relationship with idiopathic male infertility in the studied Iranian men population.
Conclusion: According to these research results, the T132903C polymorphism in the INSR gene is affected by idiopathic infertility in the Iranian male population and could cause oligospermia and azoospermia in Iranian infertile males. To confirm these results, it is recommended to study more samples.
Type of Study: Research Article |
Subject:
Genetics
Received: 2025/09/2 | Accepted: 2025/07/1 | Published: 2025/07/1
Received: 2025/09/2 | Accepted: 2025/07/1 | Published: 2025/07/1
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