Volume 16, Issue 63 (8-2026)
NCMBJ 2026, 16(63): 126-141 |
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Moti M, Ramezani M, Asgarimoghadam N, Ahmadpanahi H. Synthesis And Characterization of Polymeric Magnetic Nanocomposites Containing Myricetin, And Evaluation of Their Cytotoxic and Apoptosis-Inducing Effects in HCT116 Colorectal Cancer Cells. NCMBJ 2026; 16 (63) :126-141
URL: http://ncmbjpiau.ir/article-1-1874-en.html
URL: http://ncmbjpiau.ir/article-1-1874-en.html
Department of Biology, CT. C., Islamic Azad University, Tehran, Iran.
Abstract: (7 Views)
Extended Abstract
Background and Aim: Colorectal cancer (CRC) remains a significant global health challenge, necessitating the exploration of innovative therapeutic modalities. Myricetin, a naturally occurring flavonol, exhibits substantial antitumor potential; however, its clinical application is constrained by poor aqueous solubility and rapid metabolic clearance. This study aimed to design and characterize myricetin-loaded magnetic polymeric nanocomposites to enhance the therapeutic efficacy of myricetin against HCT116 human colorectal cancer cells.
Material and methods: Superparamagnetic iron oxide nanoparticles (Fe3O4Fe_3O_4Fe3O4 NPs) were synthesized via the chemical co-precipitation method. The surface of these nanoparticles was functionalized with a biocompatible polymer to facilitate drug loading. Physicochemical characterization was performed utilizing X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FE-SEM), Vibrating Sample Magnetometry (VSM), and Dynamic Light Scattering (DLS). The in vitro drug release profile was characterized using the dialysis membrane technique in a physiological buffer. The cytotoxic potential was evaluated using the MTT assay, while apoptotic cell death was quantified via Annexin V-FITC/PI flow cytometry.
Results: Morphological and structural analyses confirmed the successful synthesis of stable, drug-loaded nanocomposites. DLS analysis indicated a primary population peak at 95 nm with a PDI of 0.95. This polydispersity is attributed to the inherent magnetic dipole-dipole interactions common in Fe3O4-based systems. Drug release studies demonstrated a controlled (by pH and temperature), sustained-release profile, highlighting the suitability of the nanocarrier for targeted delivery. The cytotoxicity assay revealed that myricetin-loaded nanoparticles inhibited HCT116 cell proliferation in a concentration-dependent manner, with an IC50 value of 37.5 µg/mL. Furthermore, flow cytometric analysis confirmed a statistically significant increase in the apoptotic population relative to the control group, suggesting that the nanocarrier effectively facilitates myricetin internalization and pro-apoptotic activity.
Conclusion: The findings of this study demonstrate that polymeric magnetic nanoparticles serve as an efficient platform for myricetin delivery, effectively overcoming its solubility limitations and enhancing its cytotoxic effect on CRC cells. The ability of this nanodrug to induce apoptosis suggests a promising avenue for developing advanced, targeted cancer therapies. Future studies focusing on in vivo efficacy and molecular signaling pathways are warranted to elucidate the clinical potential of this nanocomposite further.
Background and Aim: Colorectal cancer (CRC) remains a significant global health challenge, necessitating the exploration of innovative therapeutic modalities. Myricetin, a naturally occurring flavonol, exhibits substantial antitumor potential; however, its clinical application is constrained by poor aqueous solubility and rapid metabolic clearance. This study aimed to design and characterize myricetin-loaded magnetic polymeric nanocomposites to enhance the therapeutic efficacy of myricetin against HCT116 human colorectal cancer cells.
Material and methods: Superparamagnetic iron oxide nanoparticles (Fe3O4Fe_3O_4Fe3O4 NPs) were synthesized via the chemical co-precipitation method. The surface of these nanoparticles was functionalized with a biocompatible polymer to facilitate drug loading. Physicochemical characterization was performed utilizing X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FE-SEM), Vibrating Sample Magnetometry (VSM), and Dynamic Light Scattering (DLS). The in vitro drug release profile was characterized using the dialysis membrane technique in a physiological buffer. The cytotoxic potential was evaluated using the MTT assay, while apoptotic cell death was quantified via Annexin V-FITC/PI flow cytometry.
Results: Morphological and structural analyses confirmed the successful synthesis of stable, drug-loaded nanocomposites. DLS analysis indicated a primary population peak at 95 nm with a PDI of 0.95. This polydispersity is attributed to the inherent magnetic dipole-dipole interactions common in Fe3O4-based systems. Drug release studies demonstrated a controlled (by pH and temperature), sustained-release profile, highlighting the suitability of the nanocarrier for targeted delivery. The cytotoxicity assay revealed that myricetin-loaded nanoparticles inhibited HCT116 cell proliferation in a concentration-dependent manner, with an IC50 value of 37.5 µg/mL. Furthermore, flow cytometric analysis confirmed a statistically significant increase in the apoptotic population relative to the control group, suggesting that the nanocarrier effectively facilitates myricetin internalization and pro-apoptotic activity.
Conclusion: The findings of this study demonstrate that polymeric magnetic nanoparticles serve as an efficient platform for myricetin delivery, effectively overcoming its solubility limitations and enhancing its cytotoxic effect on CRC cells. The ability of this nanodrug to induce apoptosis suggests a promising avenue for developing advanced, targeted cancer therapies. Future studies focusing on in vivo efficacy and molecular signaling pathways are warranted to elucidate the clinical potential of this nanocomposite further.
Keywords: Myricetin, Polymeric Magnetic Nanoparticles, Nanodrug Delivery, Apoptosis, HCT116 Cells, Colorectal Cancer
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2026/09/8 | Accepted: 2026/08/1 | Published: 2026/08/1
Received: 2026/09/8 | Accepted: 2026/08/1 | Published: 2026/08/1
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