Volume 2, Issue 8 (10-2012)                   NCMBJ 2012, 2(8): 27-35 | Back to browse issues page

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Nasiri N, Nemat zadeh G, Askari H, Shokri E. Study of Aeluropus littoralis Leaves Proteom Response to Salinity Stress. NCMBJ. 2012; 2 (8) :27-35
URL: http://ncmbjpiau.ir/article-1-252-en.html
Genetic & Agricultural Biotechnology of Tabarestan (GABIT), University of Agriculture Sciences and Natural Resources, Sari, Iran , najmeh.nasiri@gmail.com
Abstract:   (22456 Views)

Aim and Background. Due to evolving in stress habitats, Halophyte plants are the best options to describe the fundamentals of molecular physiology of salt tolerance and breeding of crops. Therefore, because of genetic relationship of Aeluropus littoralis with rice, wheat and barley, the present study was performed to investigate leaves proteome pattern changes in response to different salt treatment using proteomics methods

 Materials and Methods. To examine the effect of salinity on the Aeluropus proteome pattern, salt treatment in 3 levels (100, 200 and 300 mM NaCl) was applied . The mature leaves located in the middle part of plant were harvested for protein extraction after 21 days of salt exposure. Proteins were extracted according to TRIZOL approach and were separated by 2-DE using a nonlinear pH 4-7 IPG strips (24 cm) and 12.5 % poly acryl amide gel.

 Results. Statistical analysis revealed that among 550 repeatable detected spots, at least 95 protein spots showed a significant change during salinity levels. In addition, the most proteins with increased and decreased expression levels were obtained in comparison between control and 200 mM NaCl levels and between 200 & 300 mM NaCl treatments, respectively. Based on cluster analysis the whole responsive proteome was classified into 10 classes.

 Conclusion. Evaluation of these co-expression classes revealed that A. littoralis salinity responsive proteome follows four distinct expression patterns. These results showed that study of individual protein expression changes alone will not be fruitful. But also the identification of co-expression proteins collection and study of proteins gregarious changes pattern in response to different levels of stress was more importance and create better understanding.

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Type of Study: Research Article | Subject: Botany
Received: 2012/12/22

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