Volume 15, Issue 60 (12-2025)
NCMBJ 2025, 15(60): 1-14 |
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Mashhoori Vayghan M, Yadegar A, Saffarian P, Tajabadi Ebrahimi M, Asadzadeh Aghdaei H. Investigating the immunomodulatory effects of extracellular vesicles and cell-free liquid of the probiotic bacteria Bacillus coagulans Hammer on colon cancer cell line HT-29. NCMBJ 2025; 15 (60) :1-14
URL: http://ncmbjpiau.ir/article-1-1780-en.html
URL: http://ncmbjpiau.ir/article-1-1780-en.html
Maryam Mashhoori Vayghan 
, Abbas Yadegar , Parvaneh Saffarian , Maryam Tajabadi Ebrahimi , Hamid Asadzadeh Aghdaei
, Abbas Yadegar , Parvaneh Saffarian , Maryam Tajabadi Ebrahimi , Hamid Asadzadeh Aghdaei
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran , a.yadegar@sbmu.ac.ir
Abstract: (12 Views)
Abstract
Aim and Background: Bacillus coagulans Hammer is a Gram-positive probiotic that releases extracellular vesicles (EV). These vesicles contain the surface proteins of the bacteria, which are able to stimulate the immune system. For newborns or immunosuppressed people, the use of these extracellular vesicles is much less dangerous. As a result, the use of these vesicles causes a change and improvement in the inflammatory responses. In this study, our aim is to investigate the changes in immune responses after using extracellular vesicles and cell-free liquid of B. coagulans Hammer on HT-29 cells, as a colon cancer cell line.
Materials and Methods: B. coagulans hammer EVs were extracted using polyethylene glycol. Cell-free supernatant was prepared, and HT-29 cells were treated with a concentration of 10 μg/mL of EVs and a dilution of 20% v/v. The RT-qPCR method was used to assess the expression levels of inflammatory genes IL-1β, IL-6, IL-8, IL-10, TNF-α, and TGF-β, and the ELISA method was used to assess the levels of TGF-β and TNF-α cytokines produced in the case.
Results: The results obtained from RT-qPCR showed that the EVs and Cell Free Supernatant can reduce the expression of inflammatory genes IL-1β, IL-6, IL-8, TNF-α and increase the expression of anti-inflammatory genes IL-10 and TGF-β. TNF-α and TGF-β cytokines have been examined by the ELISA method also shows an increase in TGF-β and a decrease in TNF-α in the treated cells.
Conclusion: The results showed that the B. coagulans Hammer Extracellular Vesicles and Cell Free Supernatant are capable of increasing anti-inflammatory responses, in contrast to reducing inflammatory responses.
Aim and Background: Bacillus coagulans Hammer is a Gram-positive probiotic that releases extracellular vesicles (EV). These vesicles contain the surface proteins of the bacteria, which are able to stimulate the immune system. For newborns or immunosuppressed people, the use of these extracellular vesicles is much less dangerous. As a result, the use of these vesicles causes a change and improvement in the inflammatory responses. In this study, our aim is to investigate the changes in immune responses after using extracellular vesicles and cell-free liquid of B. coagulans Hammer on HT-29 cells, as a colon cancer cell line.
Materials and Methods: B. coagulans hammer EVs were extracted using polyethylene glycol. Cell-free supernatant was prepared, and HT-29 cells were treated with a concentration of 10 μg/mL of EVs and a dilution of 20% v/v. The RT-qPCR method was used to assess the expression levels of inflammatory genes IL-1β, IL-6, IL-8, IL-10, TNF-α, and TGF-β, and the ELISA method was used to assess the levels of TGF-β and TNF-α cytokines produced in the case.
Results: The results obtained from RT-qPCR showed that the EVs and Cell Free Supernatant can reduce the expression of inflammatory genes IL-1β, IL-6, IL-8, TNF-α and increase the expression of anti-inflammatory genes IL-10 and TGF-β. TNF-α and TGF-β cytokines have been examined by the ELISA method also shows an increase in TGF-β and a decrease in TNF-α in the treated cells.
Conclusion: The results showed that the B. coagulans Hammer Extracellular Vesicles and Cell Free Supernatant are capable of increasing anti-inflammatory responses, in contrast to reducing inflammatory responses.
Keywords: Bacillus coagulans Hammer, Probiotics, Immunomodulatory effects, Extracellular vesicles, HT-29 cell line
Type of Study: Research Article |
Subject:
Probiotic
Received: 2025/12/15 | Accepted: 2025/12/1 | Published: 2025/12/1
Received: 2025/12/15 | Accepted: 2025/12/1 | Published: 2025/12/1
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