Volume 8, Issue 29 (1-2018)
NCMBJ 2018, 8(29): 117-125 |
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Moradi M, Zare-zade Mehrizi H, Naderinezhad A, Haghirosadat B F, Hazrati Gohari M K, Fateme, et al . Study of Immobilization of Cytochrome c on the Modified Gold and GraphiteElectrode by Electrochemical Method. NCMBJ 2018; 8 (29) :117-125
URL: http://ncmbjpiau.ir/article-1-1065-en.html
URL: http://ncmbjpiau.ir/article-1-1065-en.html
Mahnaz Moradi 
, Hamid Zare-zade Mehrizi , Ali Naderinezhad , Bibi Fateme Haghirosadat , Mohammad Kazem Hazrati Gohari , Fateme , Fatemeh Hakimian , Azim Akbarzade
, Hamid Zare-zade Mehrizi , Ali Naderinezhad , Bibi Fateme Haghirosadat , Mohammad Kazem Hazrati Gohari , Fateme , Fatemeh Hakimian , Azim Akbarzade
1- Department of Biochemistry, Payam Nour University, Yazd, Iran
Abstract: (5131 Views)
Aim and Background:
Immobilized proteins and enzymes are commonly used in the medical field for diagnosis and treatment of various diseases. Due to the increasing use of immobilized enzymes and proteins in the diagnosis of disease, in this study, Cytochrome c protein was used as a useful metalloprotein in the basis studies of biosensors and Bioelectrochemistry.
Material and methods: Cytochrome c protein immobilized covalently on the surface of gold and graphite pencil electrods. Surface of gold and graphite electrodes modified by gold nanoparticle and self-assemble monolayer of 3- mercaptopropionic acid. EDC and NHS as coupling agents cause to covalent attachment of protein on the surface.
Results: Cyclic voltammetry results showed successful immobilization of Cytochrome c on the both surface. Immobilized protein on the surface of gold electrod was more stable than garaphite electrode.
Conclusion: In view of the Cytochrome c as a sample biological species, both surfaces can be suitable for immobilization of various biological species, and selection any of them depend on their application in various fields.
Immobilized proteins and enzymes are commonly used in the medical field for diagnosis and treatment of various diseases. Due to the increasing use of immobilized enzymes and proteins in the diagnosis of disease, in this study, Cytochrome c protein was used as a useful metalloprotein in the basis studies of biosensors and Bioelectrochemistry.
Material and methods: Cytochrome c protein immobilized covalently on the surface of gold and graphite pencil electrods. Surface of gold and graphite electrodes modified by gold nanoparticle and self-assemble monolayer of 3- mercaptopropionic acid. EDC and NHS as coupling agents cause to covalent attachment of protein on the surface.
Results: Cyclic voltammetry results showed successful immobilization of Cytochrome c on the both surface. Immobilized protein on the surface of gold electrod was more stable than garaphite electrode.
Conclusion: In view of the Cytochrome c as a sample biological species, both surfaces can be suitable for immobilization of various biological species, and selection any of them depend on their application in various fields.
Type of Study: Research Article |
Subject:
Biochemistry
Received: 2018/03/3 | Accepted: 2018/03/3 | Published: 2018/03/3
Received: 2018/03/3 | Accepted: 2018/03/3 | Published: 2018/03/3
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