Volume 8, Issue 32 (10-2018)                   NCMBJ 2018, 8(32): 53-64 | Back to browse issues page

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Zamani L, Zaker Bostanabad S, Salehipour M. Evaluation spirulina extract effect on DU145cell line and oct4 gene expression pattern in treated cell. NCMBJ. 2018; 8 (32) :53-64
URL: http://ncmbjpiau.ir/article-1-1137-en.html
Islamic Azad University, Parand Branch, Parand, Iran , saeedzaker20@yahoo.com
Abstract:   (4035 Views)
Aim and Background:
Prostate cancer is the second common malignancy in the male population. In this study, the effect of Spirulina alga extract on DU145 prostate cancer cell line have been studied and the changes in OCT4 gene expressionin this cell line was evaluated.
Material & methods:
 The extract of spirulina algae was affected on the DU-145(prostate cancer cell line), and the rate of attenuation of the cells were evaluated by MTT and the expression of OCT4 were evaluated using Real Time PCR. The data was analyzed by SPSS.
Results:
 The results showed that this extract could reduce significantly cell proliferation after 48 hours and significantly decrease oct4 expression. These   changes increase with increasing concentrations and exposure time. The highest expression loss was observed at 800 μm of extract in 72 hours after treatment.
Conclusion:
it can be concluded that the Spirulina extract, by acting on the main genes of the self-renewal pathway and reducing its expression, helps to reduce the amount of cell division, and this decrease leads to avoid the development of cancerous tissue.
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Type of Study: Research Article | Subject: Cellular and molecular
Received: 2018/10/13 | Accepted: 2018/10/13 | Published: 2018/10/13

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