Volume 10, Issue 38 (3-2020)
NCMBJ 2020, 10(38): 99-111 |
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Siasi E, Rezaei A, Nowroozi J. Identification of 3 Virulence Factors kpsMTII, iucD‚ usp in Urinary Tract Infection E. coli by Multiplex-PCR. NCMBJ 2020; 10 (38) :99-111
URL: http://ncmbjpiau.ir/article-1-1279-en.html
URL: http://ncmbjpiau.ir/article-1-1279-en.html
Department of Microbiology, Collage of science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Abstract: (2773 Views)
Aim and Background: E. coli is a normal flura in the human and animal intestinal tract. This bacteria is one of the main cause of the urinary tract infection. The aim of this study was identification and simultaneously presence of 3 virulence factors, kpsMTII, iucD, usp in the genome of urinary tract infection E. coli strains.
Materials and Methods: 60 samples of E. coli, which caused urinary tract infection, were collected. These bacteria were isolated by biochemical tests and gram staining. Then bacteria genome was extracted by gram-negative bacteria DNA extraction kits. Multiplex-PCR was used for identifying of 3 virulence factors.
Results: In these 60 samples of isolated E. coli, the prevalence of virulence genes is as follows: kpsMTII 71.66%, iucD 88.33%, and usp 36.66%. As also, simultaneously presence of 3 virulence factors were observed in 28.33% of samples. There was significant association between prevalence of these three genes and urinary tract infection in studied E. coli isolates (Pvalue<0.05).
Conclusion: According to this study results, that was similar to previous researches, could be significant related between prevalence of these three genes in studied urinary tract infection E. coli samples.
Materials and Methods: 60 samples of E. coli, which caused urinary tract infection, were collected. These bacteria were isolated by biochemical tests and gram staining. Then bacteria genome was extracted by gram-negative bacteria DNA extraction kits. Multiplex-PCR was used for identifying of 3 virulence factors.
Results: In these 60 samples of isolated E. coli, the prevalence of virulence genes is as follows: kpsMTII 71.66%, iucD 88.33%, and usp 36.66%. As also, simultaneously presence of 3 virulence factors were observed in 28.33% of samples. There was significant association between prevalence of these three genes and urinary tract infection in studied E. coli isolates (Pvalue<0.05).
Conclusion: According to this study results, that was similar to previous researches, could be significant related between prevalence of these three genes in studied urinary tract infection E. coli samples.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2020/05/13 | Accepted: 2020/05/13 | Published: 2020/05/13
Received: 2020/05/13 | Accepted: 2020/05/13 | Published: 2020/05/13
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