The phylogeny of Escherichia coli isolates involved in Colibacillosis and cellucitis in broiler chickens in shahrebabak  by Multiplex PCR Technique
Akbar Asadi^*1, Taghi Zahraei Salehi², Reza Ghanbarpour³_، navid asadi⁴
1. ShahrBabak Branch Islamic Azad University, Shahrebabak, Iran
2. Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran                            
3. Department of Microbiology and Immunology ،Faculty of  Veterinary Medicine ،Shahid Bahonar ،Kerman ،Iran
4. Veterinary student of Bahonar university,kerman,Iran.
Abstract
Aim and Background: Escherichia coli infections in poultry have a word wide spread. Serotypies O₁, O₂, O₈, O₃₅, O₇₈ Escherichia coli is involved in the development of generalized colibacillosis disease. Genes such as F17, Sfa, pap, afa, , fimH , crl , iuc, bla, yja, chu, are considered as an Escherichia coli acuity factoro. the aim of the present study were Phylogentic of Escherichia coli isolates involved in Colibacillosis and cellucitis in broiler chickens in shahrebabak(Kerman Province) by Multiplex PCR Technique 
Material and methods: From 117 samples of Escherichia coli (83 colibacillosis samples and 34 cellulitis samples) were isolated. These samples were examined and confirmed by biochemical methods.
Results: Colibacillosis isolates were belonged to A (54.27%), B1 (7.22%), B2 (6.03%) and D (32.53%) phylogroups. Where as, the isolates from cellulitis cases were belonged to three main phylogroups; A (55.88%), B1 (5.88%) and D (38.24%).
Conclusion: Statistical analysis showed a specific association between the presence of crl virulence gene and Phylogrups of A and D (P<0.05) in colibacillosis isolates.
The results showed that the isolates from both diseases in broiler chickens could be assigned to various phylogenetic groups (mainly A). Also, the virulence genes profile of cellulitis E.coli is completely different from that of colibacillosis in this region..
Key words: Escherichia coli, colibacillosis, phylogenetic group, cellulitis, virulence genes.