Volume 5, Issue 17 (3-2015)
NCMBJ 2015, 5(17): 8-12 |
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Fatemi F, Najafi zarrini H, Heydari P. Phylogenetic and Functional Study of SOS1 Gene to Different Plant Species. NCMBJ 2015; 5 (17) :8-12
URL: http://ncmbjpiau.ir/article-1-365-en.html
URL: http://ncmbjpiau.ir/article-1-365-en.html
University of Agriculture and Natural Resources of Sari , najafi316@yahoo.com
Abstract: (8531 Views)
Aim and Background: Soil salinity is one of the major abiotic stresses reducing yield crop. SOS (Salt overly sensitive) system in Arabidopsis thaliana act as cellular signaling system in salinity tolerance pathways. SOS1 has an important role in salinity tolerance.
Materials and Methods: In this study, SOS1 gene sequence identified in various plants has been recorded in the databases such as Tair, EBI, NCBI, plant care and EXPASY. We use an in silico analysis in order to determine phylogenetic study and the homology of 13 plant species by bioedit, UPGMA and MEGA5 software.
Results: The cluster analysis showed that sos1 protein sequences of these plants classified in three groups. The results of the evolutionary matrix showed that the most distance was between Aegilops tauschii and Arabidobsis thaliana (0.542) and the least distance was between Triticum aestivum and Aegilops tauschii (0.004). Promoter Sequence analysis of SOS1 gene showed that, the ABRE and CAAT boxes were found in all areas of plant promoter. Also, aligning protein sequences showed complete similarity in 15 Amino acids.
Conclusion: Due to the presence of ABRE box activated in response to Abscisic acid and CAAT box and increases gene expression in all areas of plant promoter, suggests the potentiality of plants to cope with abiotic stresses. Complete similarity in 15 Amino acids shows conserved region during the evolution process and can have an important structural role in the protein's function and nucleotide sequences of those suggested to design primers.
Results: The cluster analysis showed that sos1 protein sequences of these plants classified in three groups. The results of the evolutionary matrix showed that the most distance was between Aegilops tauschii and Arabidobsis thaliana (0.542) and the least distance was between Triticum aestivum and Aegilops tauschii (0.004). Promoter Sequence analysis of SOS1 gene showed that, the ABRE and CAAT boxes were found in all areas of plant promoter. Also, aligning protein sequences showed complete similarity in 15 Amino acids.
Conclusion: Due to the presence of ABRE box activated in response to Abscisic acid and CAAT box and increases gene expression in all areas of plant promoter, suggests the potentiality of plants to cope with abiotic stresses. Complete similarity in 15 Amino acids shows conserved region during the evolution process and can have an important structural role in the protein's function and nucleotide sequences of those suggested to design primers.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2013/07/29 | Accepted: 2014/08/21 | Published: 2015/02/24
Received: 2013/07/29 | Accepted: 2014/08/21 | Published: 2015/02/24
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