Aim and Background: Chronic Obstructive Pulmonary Disease (COPD) is caused by inflammation and destruction of a part of lung and usually appears after age 50. Considering the muscle weakness, energy limitation and problem with oxygen consumption in COPD patients, we analyzed the mitochondrial cytochrome c oxidase (COX) gene to find the relationship between COXI and COPD disease. We studied 65 patients (71±8 years old) who were clinically stable and 50 healthy people (56±4 years old) as a control group. DNA was extracted and prepared according to the standards. Material and method: Isolated DNA was used for amplification of COXI gene. In this study, screening four new polymorphisms using ARMS Real time PCR was performed in COXI.

Results: PCR conditions were optimized empirically for screening 4 polymorphisms by ARMS Real time PCR. Significant difference polymorphisms between COPD patient and normal control was found

Conclusion: The light note of polymorphism was found at 6060 locus, which is the only polymorphism that leads to an amino acid change. Increasing the value of this polymorphism in patients with COPD than normal individuals can make this polymorphism a risk factor in COPD