Materials and Methods. The 20 strains used in this study were isolated from different sources. Samples were grown in MRS broth then total genomic DNA was extracted. RAPD markers were used for the detection of genetic diversity.

Results. Out of 20 primers used 19 primers produced reproducible bands. The primers generated 225 RAPD loci, of which 219 were polymorph, 6 were monomorph and 63 were unique. NJ and UPGMA clustering methods performed on molecular data by NTSYS-PC software. Similar results are obtained by two methods.

Conclusion. UPGMA clustering methods were performed on molecular data. Two genotypes of k₂l₃ and y₁m₄ are grouped together in two dendrograms. In UPGMA dendrogram two major clusters A and B were observed. Cluster A was divided in to2 subcluster by c₆m₃،y₁l₄، y₂f₃ and c₁d₂ genotypes. In cluster B, 2 subclusters by 6 genotypes (c₂h₁، d₃b₁، y₂c₄، k₂l₃و y₁m₄ and p) were observed.

Key words. Lactobacillus strain, genetic diversity, traditional dairy products, RAPD markers, UPGMA dendrogram.