Aim and background:Wide application of nanoparticles in different area of medical science has been growing faster than ago. Small size makes them high active. Therefore this gives them chemical, physical and biological unique properties. Nanoparticles will have not much problem to cross through biological barriers in body. Hence we can use these particles for targeting drugs and some materials. Wide application needs to investigate their cytotoxicity effects on target cells and adjacent cells. In this study we tried to analysis effect of nanoparticles on invitro.

Materials and Methods: Related articles in this review were obtained from Pub Med, Google ,Elsiver science using the key words nanoparticles, cytotoxicity and differentiation.

Results: Base on the results of this study nanoparticles are able to leave both positive and negative effects. invitro experiments indicate that the main mechanism of action is based on changing levels of intracellular ROS.

Conclusion: The results of reported studies have shown that nanoparticles are able to alter intracellular ROS levels and affect growth and differentiation cells.