Volume 5, Issue 18 (4-2015)                   NCMBJ 2015, 5(18): 95-99 | Back to browse issues page

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Eslami G, Samadi R, Taherpor A. Frequency of Listeria monocytogenes prfA, actA , inlB genes among infertile women referred to Medical center of University by PCR Method in 2013 . NCMBJ. 2015; 5 (18) :95-99
URL: http://ncmbjpiau.ir/article-1-633-en.html
Department of Microbiology, Faculty of Medicine, Shahid Beheshti University, M.C., Tehran, Iran. , Rsamadi88@yahoo.com
Abstract:   (8722 Views)

Aim and Background: Recently the spontaneous abortion has been thea matter of attention by researchers. Therefore, bacteria such as Listeria monocytogenes, Chlamydia trachomatis, Mycoplasmas and Streptococcus agalactia are important agents of this phenomenon. When they are not recognized in due time , we found spontaneous abortion and increase of antibiotics resistance. The aim of this study was to assess the extent of L. monocytogenes in causing human spontaneous abortions by isolation methods and PCR analysis for the presence of actA, PrfA and InlB genes.
Material and Methods: In this descriptive study, vaginal swabs were collected from a total of 96 women with spontaneous abortions who referred to Tehran`s hospitals. The samples were transported in to TSBYE broth. Then, the samples were enriched at 4 ͦ C for 1 month. Total DNA of L. monocytogenes was extracted by Fermentase kit. PCR test is performed on all samples and detected the actA, PrfA and InlB genes of L. monocytogenes.
Results : In PCR technique, actA, PrfA and InlB gene were detected 12.5%, 9.3% and 2% respectively from 96 patients with spontaneous abortions.
Conclusions: The occurrence of pathogenic L. monocytogenes in cases of spontaneous abortions was 12.5%. It seems that the actA, PrfA and InlB genes have an important role as essential virulence determinant in pathogenic L. monocytogenes.

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Type of Study: Research Article | Subject: Cellular and molecular
Received: 2015/05/31 | Accepted: 2015/05/31 | Published: 2015/05/31

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