Volume 1, Issue 4 (10-2011)                   NCMBJ 2011, 1(4): 49-57 | Back to browse issues page

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Ebrahimi M A, Falahzadeh R, Norouzi P, Rajabi A. Selection of Dominant Homozygous Plants Resistant to Rhizomaniain Cultivated Beta Germplasm Using RAPD-PN3 Marker. NCMBJ. 2011; 1 (4) :49-57
URL: http://ncmbjpiau.ir/article-1-94-en.html
Department of Agricultural Biotechnology, Payam Noor University , ebrahimi_mpn@yahoo.com
Abstract:   (19898 Views)

Aim and background. In order to identify the dominant homozygous plants resistant to rhizomania within 28 sugar beet genotypes including 5 S1 populations, 16 O-type lines, two pollinators, and 5 hybrids amounting to 1551 field-grown single plants, and a repulsion-phase RAPD marker, (named PN3) were linked to rhizomania resistance.                                                            

Materials and methods. DNA was extracted from the leaves of single plants. RAPD-PCR test was carried out on the samples using the primers. According to the data, analysis of variance for the percentage dominant homozygous plants was conducted using unbalanced completely randomized design.

 

Results. The Results indicated that the genotypes were significantly different in percentage for the dominant homozygous plants. Means comparison by Duncan’s multiple range test showed that the genotypes OT5, OT11 and OT12 had the highest percentage of marker presence. Also, by applying cluster analysis the studied genotypes were classified into four clusters with the cluster B having the genotypes of the highest dominant homozygous plants.

Conclusion. It can be concluded that the PN3 marker could be used for rapid screening of sugar beet germplasm at the stages of resistant plants selection in a resistant cultivar development program.

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Type of Study: Research Article | Subject: Cellular and molecular
Received: 2011/12/7 | Published: 2011/10/15

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