Showing 12 results for Alavi
Maedeh Koohi Moftakhari Esfahani, Seyed Ebrahim Alavi, Amir Heidarinasab, Mohsen Chiani, Azim Akbarzadeh,
Volume 3, Issue 10 (6-2013)
Abstract
Aim and background: Regarding that the breast cancer is the most prevalent disease among women, Paclitaxel, an anti-cancer drug, could be used in treatment of this disease. As Paclitaxel has some adverse effects, we made use of the nanoliposomal drug delivery technology in order to reduce adverse effects and improve drug efficacy.
Materials and Methods : Certain ratios of Phosphatidylinositol choline, cholesterol and Paclitaxel were synthesized to prepare nanoliposomal paclitaxel. The mean diameter of the nanoliposomal paclitaxel was measured by the Zeta sizer device. Using dialysis, the pattern of drug release from nanoliposomes has been studied and the cytotoxicity effect of the nanoliposomal drug was finally measured with the MTT assay.
Results: Using the Zeta sizer device, the mean diameter of the nanoliposomal Paclitaxel was obtained 421.4 nanometers and its encapsulation efficiency was 91.3%. By dialysis, drug release in the nanoliposomal Paclitaxel formulation was studied within 28 hours which was 5.53%.
Conclusion . The present study showed that the cytotoxicity effect of nanoliposomal Paclitaxel is more than that of the standard form.
Seyed Ebrahim Alavi, Maedeh Koohimoftakhariesfahani, Mohsen Chiani, Amir Heidarinasab, Azim Akbarzadeh,
Volume 3, Issue 11 (9-2013)
Abstract
Aim and background:Based on confirmed reports, it is clear that breast cancer is one of the most prevalent diseases among women.Hydroxyureais one of the important drugs used in chemotherapy.despite its therapeutic propertie, this drug has many adverse effects in long-term, this study was performed in order to use the Nano drug delivery technologyusing liposomes drug deliveryfor reducingthe adverse effects and increasing therapeutic index as well.
Materials and Methods:A certain ratio of Phosphatidylinositol choline and cholesterol was synthesized in order to prepare the nanoliposome followed by adding the hydroxyurea drug. The mean diameter of nanoliposomal hydroxyurea was determined by zeta sizer system. The percent of drug released from liposome was performed by dialysis. Also, encapsulation efficiency of nanoliposomal hydroxyurea was calculated.Ultimately the cytotoxicity of formulation was probed by MTT assay.
Results:The mean diameter of nanoliposome hydroxyurea and also and its encapsulation efficiency were recorded 402.5 nanometers and 70.836 % respectively. The pattern of drug release from nanoliposomes using dialysis method showed that the drug release of nanoliposome drug within 28 hours was equal to 25.85 %.
Conclusion:This study showed that the cytotoxicity effect of nanoliposomal drug is more than that of the released drug.
Fatemeh Khodarahmi, Gholamreza Goudarzi, Abdolrazaagh Hashemi Shahraki, Nadali Alavi, Kambiz Ahmadi Angali, Mansoreh Dehghani,
Volume 3, Issue 11 (9-2013)
Abstract
Backgrounds and aim: Ambient particulate matter (PM) is considered to be an important indicator of outdoor air quality. Many adverse health effects are associated with high concentration of particulate matter in ambient air. Exposure to pollution due to bioaerosols is an almost inescapable feature of urban living throughout the world. Exposure to ambient air microorganisms is associated with wide range of adverse health effects. The aim of the present study was to determine the concentration of particulate matter and airborne bacteria in Ahvaz ambient air and investigation of the environmental parameters effects on airborne bacteria concentration.
Materials and Methods: Air sampling for presence of bacteria was conducted using a microbial air sampler (Quick Take-30, SKC, USA). Tryptic Soy Agar which is a specific media for bacterial culture was used as a media. The number of cultivable bacteria on TSA media was counted using colony counter apparatus and was expressed as colony forming units per cubic meter (CFU m-3).
Results: The mean concentration of PM10, PM2.5, PM1 and airborne bacteria at four sampling sites were 149.35, 46.58, 25.6 µg/m3 and 446.67 CFU /m3, respectively. The highest concentration was observed in the Day month (21 December to 21 June). The lowest concentration was detected in Bahman (21 June to 21 February) month.
Conclusion: It was found that the more the human activities, the denser the urban environment, the lower vegetations, the higher the bacterial concentration, which bacterial concentrations have reverse relation with temperature and UV index.
Seyed Ebrahim Alavi, Maedeh Koohi Moftakhari Esfahani, Azim Akbarzadeh,
Volume 4, Issue 13 (3-2014)
Abstract
Aim and Background: Annual, Breast cancer takes the lives of thousands of people. Nowadays, nanotechnology is the major topics of scientific societies in targeted pharmacy field. The nanotechnology helps to control time, position and velocity of drug release. Hydroxyurea used as medicine in Breast cancer treatment that the use it has side effects. Because of this, In order to reduction side effects, increase efficiency and comfort of patients used the nano drug delivery.
Materials and Methods: Certain ratios of Phosphatidylcholine, cholesterol and polyethylene glycol 2000 were synthesized and then the hydroxyurea drug was added. The mean diameter of pegylated nanoliposomal hydroxyurea was measured with the Zeta sizer device. Encapsulation efficiency and the pattern of drug release from pegylated nanoliposomes have been studied using spectrophotometery and dialysis methods. The cytotoxicity effect of the nanoliposomal drug was measured by the MTT assay.
Results
The mean diameter of pegylated nanoliposomal hydroxyurea was estimated 338.2 nanometers. Its encapsulation efficiency was 64.212±0.173 percents. The pattern of drug release from pegylated nanoliposomal drug within 28 hours was 25.85 %.
Results: of this investigation also showed that the cytotoxic properties of pegylated nanoliposomal hydroxyurea was 38.93% more than standard hydroxyurea.
Conclusion: Polyethylene glycol demonstrated the increasing solubility of drug and in turn its better contact with the target cells.
Neda Dadgar, Seyed Ebrahim Alavi, Maedeh Koohi Moftakhari Esfahani, Mohsen Chiani, Sepideh Torabi, Azim Akbarzadeh,
Volume 4, Issue 13 (3-2014)
Abstract
Aim and Background:
Nano carriers, amongst which liposomes have remarkable significance, have recently revolutionized the treatment of a large number of diseases one of which is known to be cancer. Artemisinin is one of the drugs which are used in cancer treatment, however, despite its effectiveness it has some side effects which can be reduced by using liposomes.
Materials and Methods:
In order to produce nanoliposomal artemisinin, particular proportions of phosphatidylcholine, cholesterol and artemisinin were mixed together. Afterward, the diameters of liposomes we determined by means of Zeta sizer system. The encapsulation efficiency was estimated through spectrophotometery method. The amount of released artemisinin from the formulation was measured by dialysis for 24 hours. Cytotoxicity amount of pegylated nanoliposomal artemisinin was measured by means of MTT assay.
Results:
The mean diameter of nanoliposomes was 455 nm. The encapsulation efficiency and the amount of released drug from artemisinin formulation and pegylated nanoliposomal formulation respectively were 5/3±62/91 and 17/5. The results show that the ratio of the IC50 of the prepared formulation to standard drug is a smaller amount.
Conclusions:
This study explains that the ratio of Cytotoxicity amount of this drug has been decreased compared to the standard drug, which can be achieved by nanoliposome preparation and pegylation of artemisinin.
Maedeh Koohi Moftakhari Esfahani, Seyed Ebrahim Alavi, Azim Akbarzadeh,
Volume 4, Issue 14 (4-2014)
Abstract
Aim and Background:
Formulation of anticancer drugs aroused intensive research in the past decades. Pegylated nanoliposomes have been utilized as effective drug carriers, a vehicle for drug delivery. The aim of this study is to evaluate the performance of polyethylene glycol on the liposome structure.
Materials and Methods:
In order to produce pegylated nanoliposomal certain proportions of phosphatidylcholine, cholesterol, polyethylene glycol and paclitaxel were mixed together. The mean diameter of pegylated nanoliposomal paclitaxel was determined by Zeta sizer system. The encapsulation efficiency was calculated using standard curve. The percent of drug released from pegylated nanoliposomes was performed by dialysis for 28 hours. Also, cytotoxicity of pegylated nanoliposomal paclitaxel was studied through MTT technique.
Results:
The particles’ mean diameter confirmed in nano dimensions. Encapsulation efficiency and drug release of pegylated nanoliposomal paclitaxel was 95.2±6.3% and 5.02, respectively. Results showed an increase in toxicity, the formulation was prepared.
Conclusion:
Polyethylene glycol causes more stability and slower release of drug.
Fatemeh Dashti Rahmat Abadi, Anita Abedi, Hasan Ebrahimi Shahmabadi, Maedeh Koohi Moftakhari Esfahani, Seyed Ebrahim Alavi, Adel Marzban, Azim Akbarzadeh,
Volume 4, Issue 15 (9-2014)
Abstract
Aim and Background: carboplatin as an anticancer agent belongs to platinum family drugs. Along with anticancer functionalities, this drug may have some side effects. Targeted and selective prescriptions of drugs reduce their side effects. Nanotechnology-based drug delivery systems are available approaches to overcome this problem. In this study, carboplatin was loaded on poly butyl cyanoacrylate nanoparticles and then its features were evaluated.
Materials and Methods: carboplatin was loaded into nanoparticle during polymerization of monomer. Monomer was polymerized by emulsion polymerization technique. To determine amount of loaded drug on nanoparticle, spectrophotometery method was used. Evaluation of the cytotoxicity effects of free form of drug and carboplatin-loaded nanoparticle were performed on MCF-7 cell line using MTT assay.
Results: size and zeta potential of carboplatin-loaded nanoparticles were found to be 319 nm and -7 mv. Loading percentage of carboplatin on nanoparticles was estimated 6%. Evaluation of cytotoxicity effects shown survival rate due to carboplatin and carboplatin-loaded nanoparticle at concentration 20 macro molar (p<0.01) were estimated 80±0.6% and 84±0.6%, respectively. These values at the concentration of 160 macro molar (p<0.01) were estimated %44±0.5 and %51±0.2, respectively.
Conclusion: Probably, due to low loading efficiency, cytotoxicity effects of carboplatin-loaded nanoparticle were decreased in comparison with free form. Further studies were needed to construct suitable carboplatin-loaded poly butylcyanoacrylate nanoparticle. In this way miniemulsion polymerization could be recruited.
Seyed Ebrahim Alavi, Maedeh Koohi Moftakhari Esfahani, Azim Akbarzadeh,
Volume 4, Issue 15 (9-2014)
Abstract
Aim and Background:
Carriers have made a big evolution in the treatment of many diseases in recent years. Lipid carriers are of special importance among carriers. Archaeosome is one of the lipid carriers. In this study, paclitaxel was archaeosomed to reduce side effects and improve its therapeutic index.
Materials and Methods:
In order to prepare nanoarchaeosomal paclitaxel, Archaeosomes are synthesized with a certain ratio of paclitaxel in PBS. The mean diameter of archaeosomal paclitaxel was calculated by Zeta sizer. Encapsulation efficiency of the prepared formulation was calculated by spectrophotometry. Cytotoxicity effect was determined by MTT method. Drug release pattern was determined by dialysis in 26 hours. Using artificial neural network, amount of released nanoarchaeosomal drug was predicted accurately. In this method, the release was predicted by 4 layers and 20 neurons in hidden layers.
Results:
The mean diameter of archaeosomal paclitaxel was found to be about 521.4 nm. Encapsulation efficiency of the prepared formulation was 99.1±0.21. Drug releasing of archaeosomal paclitaxel was 0.149% within 26 hours. Predicted values of release for nanoarchaeosomal paclitaxel have had R= 0.99716 and MSE=4.01 × 10-13.
Conclusion:
The used model demonstrated that artificial neural network technique can predicts the amount of release with high precision. Also it is possible to predict released amount of other drugs by this model. Although drug release has special pattern which should be considered.
Seyed Kazem Bagherpour Doun, Azim Akbarzadeh, Sohrab Halal Khor, Hasan Ebrahim Shahmabadi, Seyed Ebrahim Alavi, Mehri Mortazavi, Zahra Saffari, Maryam Farahnak,
Volume 5, Issue 17 (3-2015)
Abstract
Introduction: Cisplatin (Cispt) is an anti-cancer drug with a low level of solubility. One of Cispt`s solvents is dimethyl sulfoxide (DMSO) which can be substituted with chlorine of drug as Cispt`s solvent. Using this solvent is impossible in biological studies due to intense reduction in activity. On the other hand, it is specified that Cispt`s stability is increased in aqueous media by increasing sodium chloride (NaCl) concentration up to 0.9%. Consequently, we intended to study the effect of DMSO on cytotoxicity of Cispt in the presence of sodium.
Materials and Methods: G-292 cells and MTT assay was used to study cytotoxicity of different compounds in culture media. FTIR was employed to investigate chemical structure of Cispt and Cispt dissolved in DMSO.
Results: cytotoxicity in dilutions of 300 and 9 (p<0.01) of Cispt in Cispt+NaCl+DMSO formulation was equal to 78 and 7%. These numbers were estimated 79 and 18% for Cispt+ DMSO formulation. Studying chemical structure of Cispt and Cispt dissolved in DMSO showed that sodium chloride cannot inhibit inactivating effect of DMSO on Cispt and effect of this solvent on Cispt is independent from presence of sodium chloride.
Conclusion: This study showed that effect of solvent on Cispt is independent from presence of sodium chloride. Our findings suggest more studies to use this solvent for Cispt.
Saba Toloei, Maedeh Koohi Moftakhari Esfahani, Fatemeh Movahedi, Seyed Ebrahim Alavi, Azim Akbarzadeh,
Volume 5, Issue 18 (4-2015)
Abstract
Aims and Background back: Bacterial cellulose has properties such as higher elasticity, strength and biocompatibility and produced fibers are 100 times smaller than natural cellulose. It is applicable as three dimensional extracellular matrices for holding cells, controlling tissue structure and regulating cell activities.
Materials and Methods: Bacterial cellulose was produced by Acetobacter xylinum ATCC 10245. Then Cellulose scaffold was synthesized and characterized. Microstructures, formed bonds and crystalline structure were analyzed by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD), respectively.
Results: The fibers were synthesized with diameter of about 30 nm to 360 nm. Different pore sizes of more than one micron were observed. Fiber length was in micron scale. Thus microbial cellulose was quite finer in comparison with natural cellulose. Furthermore, the amount of synthesized bacterial cellulose was in direct relation with area of medium in contact with air.
Conclusion: Considering the results and especially existence of micron scale pores, synthesized bacterial cellulose was quite applicable in tissue engineering.
Hasan Ebrahim Shahmabadi, Rahimeh Rasouli, Seyed Ebrahim Alavi, Maedeh Koohi Moftakhari Esfahani, Azim Akbarzadeh,
Volume 5, Issue 19 (7-2015)
Abstract
Aim and Background: In recent decades, drug carriers in nano scale have been focus of attentions. In last two decades, cyanoacrylate nanoparticles have been widely studied as drug nanocarriers. In this work, production of polybutyl cyanoacrylate (PBCA) nanoparticles by emulsion polymerization has been investigated.
Material and Methods: polybutyl cyanoacrylate nanoparticles were produced by emulsion polymerization using 70 KDa dextran as stabilizer. Nanoparticles were described by Zeta sizer, scanning electron microscope and light microscope. Effect of polymerization factors on size and distribution of particles, was studied. Such factors include 70 KDa dextran, polysorbate-80, concentration of H+ ion, polymerization time, temperature and sonication.
Results: This study indicates that a definite portion of stabilizer is essential for the production of nanoparticles. This concentration was recognized to be 2%. Effect of H+ ion was considerable as a sharp decrease was observed in pH 4. In comparison with room temperature, higher size and distribution of nanoparticles was gained in temperature of 50 °C. Effect of polysorbate-80 in concentration of 0.001% on nanoparticles was evaluated to be positive. Furthermore, increasing polymerization time from 5.5 to 18 hours resulted in synthesis of more appropriate nanoparticles.
Discussion: The results illustrate that nanoparticles properties depend on various factors and manipulation of such factors can result in desirable properties.
Conclusion: PBCA nanoparticles are under the influence of various factors and we can make an acceptable and desirable nanoparticle by their proper manipulation.
Javad Shahabi, Hasan Ebrahimi Shahmabadi, Seyed Ebrahim Alavi, Maedeh Koohi Moftakhari Esfahani, Mehdi Ardjmand, Aliakbar Seyfkordi, Azim Akbarzadeh,
Volume 5, Issue 20 (10-2015)
Abstract
Aim and Background: Functional nanoparticles have offered many hopes in drug delivery. These structures not only increase the efficiency of nanoparticle-associated drugs, but also attenuate the side effects of these therapeutic agents. In this study various nanoparticulate formulations of Hydroxyurea (HU) were constructed.
Materials and Methods: reverse phase evaporation technique was used to obtain the Hydroxyurea-loaded liposome. Aspartic acid containing-Gold nanoparticles (GNPs) were manufactured using reduction of chloroauric acid salt. DNA extracted from human breast cancer cell line MCF-7 was then conjugated to GNPs (nanoconjugate). Nanoconjugate was subsequently mixed with Hydroxyurea-loaded liposome to give nanoconjugate complex. Obtained nanoparticulate formulations were characterized with dynamic light scattering (DLS) and UV-Vis spectrophotometery methods. Spectrophotometery was also used to determine the drug loading efficiency. MTT assay and MCF-7 cell line was contributed to provide the information about cytotoxicity effects of various formulations.
Results: Drug loading efficiency was found to be 70%. While nanoconjugate complex gave the largest size with 502 nm, size of produced GNPs was minimum with 29 nm. Although compared to free drug, efficiency was significantly enhanced when HU was coupled to nanoparticulate formulations, this effect was more evident at lower concentrations of drug (>20 µM). In this regard cytotoxicity effect of nanocomplex was prominent. Cytotoxicity effects of HU-loaded liposome and nanoconjugate complex at lowest concentration (5 µM) were estimated to be 70 and 81 percent respectively. More cytotoxicity observed with nanoconjugate complex could be attributed to an increase in the HU transfer to cell because of GNPs. Free HU showed cytotoxicity effects equal to 32 and 88 percent at 5 and 2500 µM, respectively.
Conclusion: Results of the study showing that liposome nanoparticle could be considered as a suitable carrier for HU. It has demonstrated that GNPs exert essential role in which at the present of this nanoparticle as nanocomplex structure, cytotoxicity effects were significantly increased. According to these observations, use of GNPs as liposomal nanocomplex for various drug formulations could be considered. Because of significance increase in the cytotoxicity effects of nanoconjugate complex produced in this study, it is recommended to initiate the in vivo studies.
