Showing 3 results for Ardjmand
Gita Bagheri, Farid Abedin Dorkoosh, Ebrahim Vasheghani-Farahani, Mehdi Ardjmand, ,
Volume 5, Issue 17 (3-2015)
Abstract
Backgrounds and Objectives: This research is to develop new systems for drug release systems based on sol - gel systems. The objective of this study is to develop controlled release formulation of water insoluble Olanzapine (OZ), using Glycerol monooleate (GMO) and Polyethylene glycol (PEG 300).
Materials and Methods: A Box- Behnken response surface methodology was applied to design gel system with 3 factors with an initial drug containing, weight ratio of GMO/water (w/w) and weight ratio of PEG 300/GMO (w/w).
Results: Therefore, in this study Design-Expert® software with Box- Behnken response surface methodology has been used specifically to determine the relationships between variables and responses.
Discussion: According to the results of experiments, a quadratic model as an appropriate equation has been selected to fit the percentage of loading. Percentage of release at 12th and 168th hr a cubic model is selected. Kinetic release profile of OZ was investigated with several models, from which Higuchi model showed the best fitting and highest correlation.
Conclusion: Optimization of gel system was carried out based on statistical concept of experimental design. Validation test was carried out under optimum conditions of the parameters predicted by the polynomial model.
Javad Shahabi, Hasan Ebrahimi Shahmabadi, Seyed Ebrahim Alavi, Maedeh Koohi Moftakhari Esfahani, Mehdi Ardjmand, Aliakbar Seyfkordi, Azim Akbarzadeh,
Volume 5, Issue 20 (10-2015)
Abstract
Aim and Background: Functional nanoparticles have offered many hopes in drug delivery. These structures not only increase the efficiency of nanoparticle-associated drugs, but also attenuate the side effects of these therapeutic agents. In this study various nanoparticulate formulations of Hydroxyurea (HU) were constructed.
Materials and Methods: reverse phase evaporation technique was used to obtain the Hydroxyurea-loaded liposome. Aspartic acid containing-Gold nanoparticles (GNPs) were manufactured using reduction of chloroauric acid salt. DNA extracted from human breast cancer cell line MCF-7 was then conjugated to GNPs (nanoconjugate). Nanoconjugate was subsequently mixed with Hydroxyurea-loaded liposome to give nanoconjugate complex. Obtained nanoparticulate formulations were characterized with dynamic light scattering (DLS) and UV-Vis spectrophotometery methods. Spectrophotometery was also used to determine the drug loading efficiency. MTT assay and MCF-7 cell line was contributed to provide the information about cytotoxicity effects of various formulations.
Results: Drug loading efficiency was found to be 70%. While nanoconjugate complex gave the largest size with 502 nm, size of produced GNPs was minimum with 29 nm. Although compared to free drug, efficiency was significantly enhanced when HU was coupled to nanoparticulate formulations, this effect was more evident at lower concentrations of drug (>20 µM). In this regard cytotoxicity effect of nanocomplex was prominent. Cytotoxicity effects of HU-loaded liposome and nanoconjugate complex at lowest concentration (5 µM) were estimated to be 70 and 81 percent respectively. More cytotoxicity observed with nanoconjugate complex could be attributed to an increase in the HU transfer to cell because of GNPs. Free HU showed cytotoxicity effects equal to 32 and 88 percent at 5 and 2500 µM, respectively.
Conclusion: Results of the study showing that liposome nanoparticle could be considered as a suitable carrier for HU. It has demonstrated that GNPs exert essential role in which at the present of this nanoparticle as nanocomplex structure, cytotoxicity effects were significantly increased. According to these observations, use of GNPs as liposomal nanocomplex for various drug formulations could be considered. Because of significance increase in the cytotoxicity effects of nanoconjugate complex produced in this study, it is recommended to initiate the in vivo studies.
Mahrokh Babaei, Majid Hasanzadegan Rudsari, Nilofar Rashidi, Mehdi Ardjmand, Azim Akbarzadeh Khiyavi,
Volume 6, Issue 22 (4-2016)
Abstract
Aim and Background: Nowadays, using nanocarriers has increased in drug delivery industry. Niosome is one of the nanocarriers with non-ionic surfactant which increases the stability of drug in body. Since, using cisplatin in the treatment of cancers has some side effects, it was nanoniosomed.
Materials and Methods: Nanoniosomes were synthesized by reverse phase evaporation and ether injection methods. The mean diameter of particles was determined using Zetasizer. The encapsulation and drug loading efficiency of two formulations which were prepared from both reverse phase evaporation and ether injection methods was measured by spectrophotometric method. Drug release pattern was examined by dialysis in a period of 48 hours. Cytotoxicity effect on nanoniosomal cisplatin was determined on C6 cell line by MTT assay.
Results: The mean diameter of particles was synthesized by reverse phase evaporation and ether injection methods was determined 242.1±5.10 nm and 218.6±3.42 nm, respectively. The encapsulation and drug loading efficiency of two formulations were measured 43.6±4.59%, 52.8±3.22%; 4.36±0.08% and 5.28±0.17%, respectively. The results of drug release showed that released drug from prepared formulation using reverse phase evaporation was 97.53±0.55% and using ether injection was 81.23±1.24%. In addition, the results of cytotoxicity showed that the cytotoxicity of prepared formulation using ether injection more than prepared formulation applying reverse phase evaporation.
Conclusion: This study showed that using reverse phase evaporation to synthesize nanoniosomal cisplatin is more appropriate method than ether that of injection method.
