Roghiyeh Samadi, Gita Eslami, Neda Baseri, Roghayeh Zeynalfam, Roya Zeynali,
Volume 4, Issue 16 (12-2014)
Abstract
Aim and Background: Extended spectrum β-lactamases (ESBLs) have emerged as a major threat worldwide with limited treatment options. This study aimed to determine the antibiotic susceptibility pattern and the evaluation of TEM genes producing in Escherichia coli and Klebsiella pneumonia isolates collected from clinical samples.
Materials and methods: Bacteria were isolated and identified from the different samples in patients sent to laboratory of shahid Beheshti University in Tehran in 2011. Isolates were then tested for antimicrobial susceptibility by disc diffusion and examined for TEM genes production by polymerase chain reaction using specific primer.
Results: Out of 100 studied nosocomial infection specimens, 50 isolates were klebsiella pneumonia of which 34 percent were ESBL producer and all were positive for TEM gene, resistance of Cefotaxime was 90 percent which is the highest degrees of resistance and lowest degrees of resistance to Imipenem was 4 percent.
Among the 50 isolates of Escherichia coli of which 14 percent were ESBL producer and all were positive for TEM gene, resistance of Cefexime was 90 percent which is the highest degrees of resistance and lowest degrees of resistance to Meropenem was 6 percent.
Conclusion: Due to relatively high prevalence of ESBL-producing bacteria in the studied population, antibiogram test are advised for appropriate treatment.
