Shaghayegh Shafighi, Mohammad Zaefizadeh, Behbood Jaafari, Mohammad Reza Alivand, Saeid Ghorbian,
Volume 14, Issue 54 (5-2024)
Abstract
Aim and Background: Breast cancer is the most common cancer in women, which is the main cause of cancer-related death in women. The use of herbal compounds as anti-tumor with less side effects than chemotherapy has been considered. But it seems that over time drug resistance will reach this type of material. This study has been conducted to determine the effect of plant extracts and exosomes extracted from their effects on MCF7 cancer cells.
Material and methods: In this comparative study, MCF7 cells were cultured in medium and treated with different concentrations of oleuropein, quercetin, coumarin and Valproic acid for 24 hours. Then the cell viability was evaluated by MTT method and its IC50 values were calculated and exosome was extracted from the culture medium of the treatments and applied to MCF7 and their IC50 values were also calculated by MTT method.
Results: The results of MTT confirmed the inhibitory properties of four natural substances and their combination on MCf7 cells. Coumarin and quercetin were the most effective treatments of the studied materials. But the exosome obtained by applying 50% of their concentration had an inhibitory role with higher concentrations and the exosomes obtained from their IC75 actions had played their role in lower inhibitory concentrations.
Conclusion: Inhibition of breast cancer cells by the exosomes obtained from the combination of the above-mentioned 4 substances occurred in a lower dose Because at this concentration, all cell death-inducing substances and their signals accumulate in the exosome, and by transferring into cancer cells, they induce cell death.
