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Aim and Background:
Liposomal vesicles provide the possibility of loading lipophilic drugs into phospholipids bilayers and water-soluble (hydrophilic) aqueous phase. The present study was aimed on the evaluation of the properties of liposomal doxorubicin loaded into liposome by both active and passive procedures in terms of drug loading and release.
Material and methods:
Dipalmitoyl glycerol phospholipid glycerol, cholesterol and DSPE-mPEG 2000 was used for synthesis of nanoliposome. Then, doxorubicin loading was performed by passive (thin film hydration) and active (according to pH gradient) methods. The average diameter of nanoparticls was measured with Zeta Sizer. And the amount of drug loading and release was performed using dialysis.
Results:
The average size of nanoparticles were 138.6 ± 4.9 nm  and 105.9 ± 3.8 nm for thin film hydration method, and pH gradient method, respectively. And drug loading efficacy of doxorubicin-containing nanoliposomes was 89±4.35 for pH gradient method and 15.65±8.65 for thin film method. The accumulated amount of drug release during 48 hours in PBS at pH=7.4, was determined 78% and 24% for hydration thin film method pH gradient method, respectively.
Conclusion:
This study shows that nanoliposomal doxorubicin prepared by active method, was more effective than that the passive method. Our prepared nanoformulation was also sensitive to pH of medium.

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Aim and Background: Because of high half- life of niosomes in blood plasma makes the possibility of delivery of chemotherapy agent to tumor tissue and improves the therapeutic index of chemotherapy drug. In this study, the nano-nisomes contained doxorubicin was synthesized. The effective dose of drug was determined and anti-cancer effect of resulting nanoparticle was evaluated. 
Material & Methods: Niosome containing cholesterol, span 60 with the 85:15 molar ratios were prepared by thin film hydration method. In the hydration step, various concentration of doxorubicin diluted with distillated water were used to determine the optimized drug concentration. The cellular cytotoxicity was finally examined using MTT assay.
Results: The results imply that optimized drug dose is 0.5mg/ml. The entrapment efficiency; size diameter and polydispersity index of optimal formulation are 81.69, 102.9 and 0.128, respectively. The amount of drug release is 35% during 144 hours. The prepared system reduces the side effects of doxorubicin and be effective against cancer cells.
Discussion: This study showed that the optimal dose of drug plays an important role in improving the percent of drug loading that is economically optimal. Also it leads to expose the patient's body with the lower doses of medication and the most therapeutic effect. Reduce the dose of medication also causes less damage to healthy cells. Sustained-release property of system is the main reason for the increased toxicity of chemotherapy drug.
Conclusions: Prepared niosomal system is slow release with size diameter less than 150 nm and high drug entrapment efficacy that can be used to overcome the side effect of free doxorubicin. The resulting system is effective against bone marrow cancerous cells.

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Aim & Background: Cancer is a major cause of mortality in the world. Treatment of Cancer through drug therapy/chemotherapy faces the challenges of drug delivery. Drug delivery systems such as niosomes provided high efficiency and targeted treatment. The aim of this study was to prepare a new formula of niosomal nano system containing doxorubicin as the cancer therapy drug. Changing the tissue distribution of drug leads to drug effect improvement and reducing its cytotoxicity.
 
Materials & Methods: Doxorubicin encapsulated niosomes were synthesized using thin-film method. A certain amount of Span-60 and Cholesterol dissolved by chloroform in a round bottom balloon. Rotary evaporator was used in order to remove the organic solvent and form the thin-film. The thin-film was hydrated by doxorubicin and PBS solution using rotary evaporator in 10°C higher than span-60 transition phase. Entrapment Efficiency and release profile were investigated using dialysis and spectrophotometry. Size reduction was applied by probe sonicating. Nanoparticles average diameter and zeta potentiol was measured using DLS technique and Zeta sizer. The optimum formula was PEGylated and its cytotoxicity investigated through MTT assay on Ovarian cancer cell line and fibroblast normal cells.
 
Results: The diameter of optimized and PEGylated niosomal formulation was 172.5 nm and entrapment efficiency of PEGylated doxorubicin system was about 95.83±1.18. release studies result in PBS buffer at 37°C was the sign of PEGylated nanoniosomes efficiency in controlling the drug release, which the release percent of drug after 48 hours was 20.52 and its cytotoxicity was higher than the free drug.
 
Conclusion: The study demonstrates using of drug delivery carriers such as synthesized nano-niosomes has an effective role in increasing the efficacy and reducing the consumed dosages of drug.