Search published articles


Showing 2 results for Sds-Page
Structural Comparison between Collagen Isolated from Sea Cucumber Holothuria Parva and Calf Skin Collagen Type I
Nasim Adibzadeh Sereshki , Saeed Aminzade, Shahla Jamili, Pargol Ghavam Mostafavi,
Volume 2, Issue 6 (6-2012)
Abstract

Aim and Background. Collagen has a wide range of applications in biomedical, pharmaceutical, cosmetic, and food industries. Recently, isolation of collagen from marine species has been investiga ted. Collagen from cow has a risk for contamination from bovine spongiform encephalopathy (BSE ), transmissible spongiform encephalopathy (TSE ). The aim of this investigation is the extraction of collagen from body wall of sea cucumber (Holothuria parva) and comparison with collagen Type I.

Material and Methods. Pepsin-solubilized collagen (PSC) is extracted from body wall of sea cucumbers (Holothuria parva) and characterized by SDS-PAGE , DSC, FT-IR.

Results. SDS-PAGE shows that the major component consists of three α1 chains of approximately 130 kDa that is similar to calf skin collagen Type I with the same weight but different in α1 chains (α1)2 α2 . The Tm of PSC is 46.940C therefore the thermal stability is lower than collagen Type I. Two peaks of transmission bands is determined by FT-IR including amide A (3420 cm-1) and amide I (1638 cm-1).

Conclusion. Collagen isolated from the sea cucumber determines Type I and the thermal stability shows that it can be used as an alternative to mammalian collagen in the food and pharmaceutical industries. The thermal stability of PSC shows that it has lower content of Proline and Hydroxyproline in comparison with collagen Type I.  


Evaluation of rhamnosyl transferase enzyme expression in the bio surfactant production pathway of Pseudomonas aeruginosa bacteria in the presence of SDS-based iron nanostructure
Yaser Ahsani Arani, Zahra Noormohammadi, Behnam Rasekh, Fatemeh Yazdian, Hojjat Kazemi ,
Volume 13, Issue 49 (12-2022)
Abstract
Aim and Background: Rhamnolipids are one of the most important bio surfactants of glycolipids produced by Pseudomonas aeruginosa. In this study, the expression of bio surfactant producing proteins of Pseudomonas aeruginosa in the presence of sodium dodecyl sulfate coated iron nanostructure (Fe/SDS) were evaluated.
Materials and Methods: The expression of Surfactant Protein A enzyme was investigated by SDS-PAGE and Western blot. The enzyme rhamnosyl transferase was analyzed on SDS-PAGE gel and confirmed by Western blotting. The presence of nanoparticles on the surface of bacteria and the morphology of nanoparticles were investigated by TEM and SEM.
Results: According to SEM, nanoparticles with a size of 20 nm and a spherical shape were seen. Binding of Fe / SDS nanoparticles to bio surfactant was confirmed by TEM. The enzyme rhamnosyl transferase with a molecular weight of 40 kDa was expressed in the test sample. The results of protein expression showed that the confirmed of rhamnosyl transferase enzyme increased significantly under the influence of Fe/SDS nanoparticles and showed the confirmed compared to other nanoparticles and the control sample. While SDS and Fe nanoparticles alone are not effective in confirmed this protein and are almost identical to the control sample. Western blotting results of SDS-PAGE confirmed the expression of rhamnolipid transferase under the influence of Fe/SDS nanoparticles.
Conclusion: The results of the present study indicate the importance of Fe/SDS nanoparticles in increasing the expression of rhamnolipid transferase enzyme in the production of bio surfactants from Pseudomonas aeruginosa.
 
Page 1 from 1     

© 2025 CC BY-NC 4.0 | New Cellular and Molecular Biotechnology Journal

Designed & Developed by : Yektaweb