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Aim and Background: Due to the high rates of diabetes in Iran and the world, as well as due to the lower side effects of medicinal plants in comparison with chemical and industrial drugs, this study investigated the effect of the active ingredient of Mary Magenta (Silybum marianum) on the expression of the Neuro D1 gene, one of the genes of the genes And the restoration of beta and alpha pancreatic cells is also a study of blood glucagon levels in rats.
Material and methods: 42 male Wistar rats were randomly selected and divided into seven groups of six. Diabetic rats received different doses of silymarin and metformin every other day for 1-3 days and after 40 days anesthetized with chloroform. After blood sampling, the serum glucagon level was measured by glucagon assay kit ELISA Then, the stages of the description and operation of pancreatic tissue extraction and extraction of RNA and cDNA were performed and expression analysis was performed using Real Time RT-PCR technique.
Results: The results of this study showed that STZ causes the destruction of pancreatic tissue and type 1 diabetes. Application of Silymarin improved diabetes and decreased glucose levels and increased serum glucagon levels in rats receiving different doses of silymarin compared to the control group (diabetic). Also, the expression level of NeuroD1 gene increased significantly compared to the GAPDH gene (reference gene).
Conclusion: The expression of NeuroD1 gene in STZ-induced diabetic rats showed a significant increase (P-Value = 0.000), which resulted in improved pancreatic tissue and decreased blood glucose and increased rats and muscle mass by regenerating cells The pancreatic alpha, as well as the binding to the promoter of the glucagon gene, enhance the expression of this gene.

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Aim and Background: Diabetes mitigates beta cells and impairs their function. Silymarin improves this disorder and decreases and also significantly increases the expression of insulin. The purpose of this study was to evaluate the effect of Silymarin on blood glucose and expression Hnf4a gene is in streptozotocin-diabetic male rats.
Material and methods:  In this research, 42 male rats were randomly selected and divided into 7 groups of heparin, diabetic rats with diabetes mellitus and streptozotocin, and their fasting blood glucose was measured. Then, doses of 50, 100, and 150 were given to the Marin's slider recipients.The rats were then anesthetized with medication. A portion of the pancreatic tissue was isolated and used to evaluate the expression of Hnf4a gene using Real Time RT PCR.
Results: Investigating the expression of Hnf4a expression at 150 mg/kg doses in rats receiving silymarin showed that in this dose significant reduction of Hnf4a gene was introduced as an effective dose (P-value <0.05). In the future, silymarin can be considered as a Herbal medicine used to treat diabetes.
Conclusion: By studying the results of this study, it can be said that the silymarin plant has anti-inflammatory effects and can be effective in reducing diabetes mellitus.

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Aim and Background: Today, research has shown that medicinal plants have fewer side effects than industrial and chemical judges. On the other hand, studies indicate the important role of Hnf1a gene in diabetes, so the aim of this study was to investigate the effect of silymarin on blood sugar and expression of Hnf1a gene in male Wistar rats diabetic with streptozoto.
 
Material and methods:  42 male rats were randomly selected and divided into seven groups. Sixty diabetic rats with diabetic streptozotocin have been measured and their fasting blood glucose was measured. After the end of the period, the rats were anesthetized with the drug. A portion of the pancreatic tissue was isolated and used to evaluate the expression of Hnf1a gene using Real Time RT PCR..
 
Results: Evaluation of Hnf1alpha at a dose of 150 mg / kg in silymarin-receiving rats showed a significant decrease in Hnf1alpha gene expression and was introduced as an effective dose (P-Value <0.05) due to the use of silymarin in the country. It is not common, in future research, more importance can be given to the effects of silymarin and it can be considered as an effective drug.
 
Conclusion: Based on the results of this study, it can be concluded that silymarin reduces blood sugar in diabetes and, if confirmed in extensive studies, can be considered as a therapeutic target in diabetes.