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Gholamreza Bakhshi Khaniki, Mina Ghasemi, Ebrahim Bairamizadeh,
Volume 1, Issue 4 (10-2011)
Abstract

Aim and Background. Ant hurium andreanum is a perennial herbaceous plant, and an economically important genus of Araceae. Tissue culture of Anthurium andreanum offers an alternative tool for rapid multiplication in a short period.

Materials and Methods. Leaf plate explants of pink cultivar were used. The leaves were cut into 1×1 cm sections. Segments of leaf were sterilized with 1% NaClO for 20 minutes. Explants were cultured on MS medium fortified with IAA (0.1- 0.4 mg/l) and BA (0.25 -1.5 mg/l), 30 g/l sucrose and 8 g/l Agar for callus induction in dark conditions. The experiment was conducted as a completely randomized design (CRD) with factorial arrangement and 8 replications. Callus was cultured on MS and ½ MS without hormones base media for shoot induction in light condition. The shoots transferred to rooting media after regeneration.

Results. Best result for callus induction was obtained from MS medium containing supplement of 0.2 mg/l IAA and 1 mg/l BA. Also the ½ MS media showed the best result for a number of shoots. The Best result for rooting in vitro was in ½ MS medium with 1g/l activated charcoal without hormones.

Conclusion. All shoots formed roots after 30 days and Plantlets were transferred into pot with perlite bed and grown in the greenhouse.


Saeed Zaker Bostan Abad, Gholamreza Bakhshi Khaniki , Fatemeh Mohsennejad,
Volume 2, Issue 5 (2-2012)
Abstract

Aim and Background. The Juglans regia L. is a plant with difficulty in rooting, therefore, its asexual reproduction is almost impossible. The aim of this study was to conduct and improve the advantage of asexual reproduction by using growth regulators of  IBA and 2,4-D.

Materials and Methods. In order to keep major characteristics, we studied the effects of treatments and growth regulators of  IBA and 2,4-D in several concentrations on 40 plantlet in five groups.

Results. The results showed that application of IBA has important  effects on root primordium initiating  but application of 2,4-D does not show significant initiation. Both treatments of IBA and 2,4-D tend to increasing length of roots.

Conclusion. This research show that,  treating of shoots with IBA  have not important effect on shoot primordium initiation,  but  2,4-D treatment are very important effects. Over all treatment with IBA and 2,4 –D caused to increasing the rooting  in survival of cutting.  



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