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Showing 7 results for Tumor
Synthesis and antitumor evaluation of spiro quinoxaline-pyrazoline derivative on K562 cell line
Maryam Bikhof Torbati, Masoud Shaabanzadeh, Mojdeh Safari, Tahereh Soleymani Ahoee, Fatemeh Khosravi,
Volume 3, Issue 10 (6-2013)
Abstract

Aim and Background: The quinoxaline and pyrazoline derivatives have variety of biological effects, which made them useful pharmaceutical agents, such as cytotoxicity activities, that will be appropriate in chemotherapy of tumors. In this study, 5'-phenyl-2',4'-dihydrospiro[indeno[1,2-b]quinoxaline-11,3'-pyrazole] compound was synthesized and studied cytotoxic effect on K562 cell line and PBMC by MTT assay. 

Materials and Methods: 5'-Phenyl-2',4'-dihydrospiro[indeno[1,2-b]quinoxaline-11,3'-pyrazole] compound by one pot assay was synthesized and studied anti tumor activity on blood cancer cell line ,K562, and healthy human hole blood activated PBMC by MTT assay. In addition, cytotoxic effects of cisplatin on the same cells were studied.

Results: This compound showed antitumor activity with IC50 of 0.92 and 0.75 µg/ml respectively on K562 cell line and PBMC+PHA. While cisplatin, as a kind of standard drug in chemotherapy, inhibited proliferation of the same cells respectively with IC50, 1.71 and 7.8 µg/ml.

Conclusion: The obtain results showed compound 4 is cytotoxic more than cisplatin on K562 cell line and activated PBMC by PHA.


Preparation of a labeled neurotensin analog and its biological evaluation for tumor imaging
Nakisa Zarrabi Ahrabi, Mostafa Erfani, Kazem Parivar, Davood Beiki, Amir Reza Jalilian,
Volume 3, Issue 10 (6-2013)
Abstract

Aim and Background. The biological actions of the peptides are mediated upon binding with high affinity to specific receptors. Neurotensin is a tridecapeptide localized in central nervous system and in peripheral tissues. Three different receptor subtypes have been described for it among which the first one showing high affinity binding for NT was observed in ductal pancreatic adenocarcinoma. Since pancreas carcinoma is among the most frequent causes of cancer deaths, the need to use new radiopharmaceuticals which are able to diagnose cancer in the early steps is evident.

Materials and Methods. A neurotensin analogue modified at the N-terminus by tetramine was obtained by solid phase peptide synthesis technique and s tandard fmoc strategy. Peptide conjugate was radiolabeled with 99mTc and s tability was tested in vitro in saline and human serum. Binding and internalization studies were carried out in neurotensin receptor expressing HT-29 cells. In vivo distribution studies were performed in normal mice.

Results. The radiolabeled complex prepared at high specific activities and >90% radiochemical yield. Peptide conjugate showed specific binding to neurotensin receptors . The radioligand showed high and specific internalization into HT-29 cells (22% at 4 h). In vivo distribution studies in mice have shown a low uptake of radioactivity in organs , such as the blood, bone, spleen, pancreas and muscle at 4 h post injection. A high uptake was found in the intestine, colon and kidneys. 

Conclusion. D ata show that this new labeled analogue exhibits favorable biodistribution pattern and is a specific radioligand for diagnostic of neurotensin receptor positive tumors .   


Evaluation of Anti tumor properties of indoloquinazoline on MCF-7 cell line
Maryam Bikhof Torbati, Masoud Shaabanzadeh, Somayeh Azadian,
Volume 6, Issue 21 (1-2016)
Abstract

Aim and Background: Chemotherapy is a category of cancer treatment that uses chemical agents. The harmfulness of chemotherapy on normal cells by killing cells is the most common side-effects of chemotherapy. Characterization and selection of new powerful chemotherapy agents which have low side effects are important. Indoloquinazoline has a variety of biological activities such as antimicrobial activities and cytotoxic properties. In this study, cytotoxic effect of synthetic indoloquinazoline compound was studied on breast cancer cell line and compared with cisplatin.

Materials and Methods: Anti tumor activity of indoloquinazoline compound and cisplatin was studied on breast cancer cell line, MCF-7, by MTT assay.

Results: Indoloquinazoline compound showed antitumor activity with IC50 of 6.83 µg/ml by being Compared with 39.13 µg/ml of cisplatin, as a kind of standard drug on MCF-7 cell line.

Conclusion: The results showed indoloquinazoline is cytotoxic more than cisplatin on MCF-7 cell line.


Application of bacteria in cancer therapy
Hajar Yaghoobi, Mojgan Bandehpour, Bahram Kazemi,
Volume 6, Issue 24 (10-2016)
Abstract

Resistance to anti-cancer therapy in patients with advanced solid tumors reveals the need for new treatments.  Major challenges of Anti-cancer therapy is, specificity of treatment. Treatment will be ideal if cancer cells be eradicated without side effects on normal cell.

 Bacteria are used in different ways to treatment of cancer, either to provide direct tumoricidal effects or to deliver tumoricidal molecules .there are two ways to use bacteria as vectors, included, tumor-specific bacterial replication and  intracellular plasmid transfer. On the other hand, Bacterial toxins act by killing the cells and control of cellular processes for example cell proliferation, differentiation and apoptosis that they are associated with cancer. Spores are very resistant bacteria that survive even in oxygen-rich conditions, but, they are not able to grow. In conditions tumor the spore grow and germinate without affecting on normal cells. Bacteria and immune system: bacterial DNA vaccines apply immune system against cancer cells via cancer antigen Presentation. Here, we review the Different applications of bacterial therapy, mechanisms of action and successes at preclinical and clinical levels. Further developments in these studies will add a new dimension to cancer treatment.


Frequency of Single Nucleotide Polymorphism of TNF--α Gene (rs1800629) in Iranian Patients with Hepatitis
Narges Bahiraei , Seyed Mehdi Sadat, Fahimeh Baghbani -Arani,
Volume 7, Issue 26 (4-2017)
Abstract
Aim and Background: Recent studies have claimed that the genetic variation in cytokine production systems has a major effect on immune system potency and strongly associated with the antiviral treatment response. On the other hand, tumor necrosis factor-alpha (TNF-α), a multifunctional pro-inflammatory cytokine, plays an essential role in host immune response to HCV infection. Therefore, current study was done in order to determine the distribution of the rs1800629 (A/G) polymorphism in the promoter of TNF-α gene in Iranian population. Material and methods: This cross-sectional study, performed on 165 blood samples based on 89 HCV infected patients including 68 SVR positive and 21 negative and 76 healthy individual controls. After DNA extraction the frequency of rs1800629 (A/G) polymorphism was analyzed using PCR-RFLP method. For detection and analysis of the PCR products we used 20% Polyacrylamide gel electrophoresis. Results: Based on analysis of the data, the distribution of the A/G polymorphism between healthy individuals and patients were obtained as AA: 1.1%, AG: 52.6%, GG: 46.1%, and AA: 1.3%, AG: 20.2%, GG: 78.7% respectively. The GG genotype was identified in 70 patients of whom 55 achieved SVR, while the AG heterozygous was found in 18 patients and SVR was achieved in 12. Finally, the AA was detected only in one patient with positive SVR. Conclusion In this study, significant difference between the TNF-α-308 locus SNP (rs1800629) and susceptibility to chronic HCV infection in Iranian population (P value= 0.002) was observed. Moreover, the presence of G allele among SVR positive people and non-SVR group did not show strong statistic difference (P value=0.476). However, further studies with more samples seems to be necessary.
Preparation and purity determination of radiolabeled taxol and evaluation of its absorption in murine melanoma
Zahra Shabani , Mostafa Erfani, Mojtaba Shamsaei Zafarghandi , Seyed Pezhman Shirmardi ,
Volume 9, Issue 34 (4-2019)
Abstract
Aim and Background:Taxol is a known substrate for P-glycoprotein, which induces drug resistance to cancerous cells. Therefore radiolabeled taxol can be a suitable probe for imaging of the P-glycoprotein transport ability to determine drug resistance. The aim of this study was to provide labeled taxol and to determine the rate of uptake in the tumor to evaluate the location of the tumor and the extent of tumor resistance to the drug.
 
Material and methods:Taxol was radiolabeled with 99mTc by direct labeling method  and after that radiochemical purity of complex was investigated. The stability and biodistribution of radiocomplex in the mice bearing melanoma tumor was performed.
 
Results:The radiocomplex was prepared with radiochemical purity more than 90%. The complex was stable (68.64%) up to 48 h. The biodistribution results showed high tumor uptake (4.51%ID/g) for radiocomplex.
 
Conclusion:High uptake of the complex in the liver as a metabolic and excretory organ and a related tumor was observed. The accumulation of radioactivity in the tumor indicates that the cells in the melanoma tumor are susceptible to taxol absorption and it is possible to detect the sites involved with the tumor using this radiocomplex. In general, based on the findings, it can be concluded that the compound has binding affinity  to the tumor and, despite the biliary excretion, can be used as a diagnostic radiopharmaceutical.
 
 
Calcalation of brain and thyroid radiation scattered absorbed dose due to proton therapy of eyes using MCNPX simulation code
Shookuh Safaiean, Mehdi Salehi Barough, Seyed Pezhman Shirmardi,
Volume 10, Issue 38 (3-2020)
Abstract
Aim and Background: Proton therapy is most  advanced treatment telepathy. protons evacuates  maximum energy in position called Bragg peak. Therefore, to treat  cancer, the tumor must be placed at the Bragg peak or SOBP. scattered photons and neutrons is a challenge in proton radiotherapy. purpose this study was to calculate dose of scattered photons and neutrons from eye in proton therapy .
Materials and Methods: In this study, simulations was performed based on MCNPX code. For this, a spherical tumor with 0.6 cm diameter is considered in the eye phantom at a depth of 2.5 mm and bragg peak is formed excactly at the end of tumor. The eye phantom is irradiated with a proton with energy  50MeV.
Results: results show that  dispersed dose of  neutrons and photons due to proton therapy  in eye tissue are 8.817e-14 Gy and 1.653e-13 Gy respectively, and dose of the esophagus and thyroid are 1.037e-07 Gy and 2.89e-08 Gy for the dispersed neutrons respectively, and for photons 5.11e-08 Gy and 1.58e-07 Gy.
Conclusion: The results show that  in the esophagus and thyroid is insignificant dispersal dose . The scattered dose of thyroid is  higher than that of esophagus, due to the proximity of this tissue to the radiation site. The results showed that less than 1% o total  proton dose  was dispersed  to other tissues.
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