---

Aim and Background: Colorectal cancer is the third most common cancer after lung cancer, stomach and liver cancer in the world. Studies have shown that patients with inflammatory disease of the large intestine are at high risk of developing colorectal cancer are located. Several factors make inflammatory bowel disease and colorectal cancer is one of the factors involved, bacteria and toxins derived from them. Research shows that some strains of E. coli can be induced to increase the mutation rate that was established in 2010 by Cuvas-Romas and colleagues. fimC and vat1 Virulence genes in these organisms play an important role in bacteria pathogenicity. This study is to compare the virulence genes in E .coli strains isolated from samples biopsy patients' inflammatory bowel disease and colorectal cancer, intestinal tissue was performed.

Materials and Methods: 38 biopsies were obtained from intestinal tissue and E.coli bacteria in microbial and biochemical method was identified and isolated. After DNA extraction, strains for virulence genes vat1, fimC using polymerase chain reaction (PCR), were evaluated.

Results: Molecular analysis showed a significant difference between the groups studied the gene vat1 (p = 0.0245) and 42.8% of positive samples were for this gene in normal groups, 87.5% with inflammatory bowel disease87.5 % of patients with colorectal cancer were reported. But there is no significant difference between the groups in terms of fimC gene (P = 0.201). And 71.4% of positive samples for this gene in normal groups' and 93.7% in patients with inflammatory bowel disease73.3 % of patients with colorectal cancer were reported.

Conclusion: The results, to confirm the good relationship between virulence genes studied induced inflammation and proliferation by inducing mutation rate.

---

Aim and Background: E. coli is a normal flura in the human and animal intestinal tract. This bacteria is one of the main cause of the urinary tract infection. The aim of this study was identification and simultaneously presence of 3 virulence factors, kpsMTII, iucD, usp in the genome of urinary tract infection E. coli strains.
Materials and Methods: 60 samples of E. coli, which caused urinary tract infection, were collected. These bacteria were isolated by biochemical tests and gram staining. Then bacteria genome was extracted by gram-negative bacteria DNA extraction kits.  Multiplex-PCR was used for identifying of 3 virulence factors.
Results: In these 60 samples of isolated E. coli, the prevalence of virulence genes is as follows: kpsMTII 71.66%, iucD 88.33%, and usp 36.66%. As also, simultaneously presence of 3 virulence factors were observed in 28.33% of samples. There was significant association between prevalence of these three genes and urinary tract infection in studied E. coli isolates (Pvalue<0.05).
Conclusion: According to this study results, that was similar to previous researches, could be significant related between prevalence of these three genes in studied urinary tract infection E. coli samples.

---

Aim and Background: Escherichia coli infections in poultry have a word wide spread. Serotypies O₁, O₂, O₈, O₃₅, O₇₈ Escherichia coli is involved in the development of generalized colibacillosis disease. Genes such as F17, Sfa, pap, afa, , fimH , crl , iuc, bla, yja, chu, are considered as an Escherichia coli acuity factoro. the aim of the present study were Phylogentic of Escherichia coli isolates involved in Colibacillosis and cellucitis in broiler chickens in shahrebabak(Kerman Province) by Multiplex PCR Technique 
Material and methods: From 117 samples of Escherichia coli (83 colibacillosis samples and 34 cellulitis samples) were isolated. These samples were examined and confirmed by biochemical methods.
Results: Colibacillosis isolates were belonged to A (54.27%), B1 (7.22%), B2 (6.03%) and D (32.53%) phylogroups. Where as, the isolates from cellulitis cases were belonged to three main phylogroups; A (55.88%), B1 (5.88%) and D (38.24%).
Conclusion: Statistical analysis showed a specific association between the presence of crl virulence gene and Phylogrups of A and D (P<0.05) in colibacillosis isolates.
The results showed that the isolates from both diseases in broiler chickens could be assigned to various phylogenetic groups (mainly A). Also, the virulence genes profile of cellulitis E.coli is completely different from that of colibacillosis in this region