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Aim and Background: Listeria monocytogenes is known as a food-borne pathogen responsible for causing disease in humans. This bacterium that cause meningitis, abortion and food poisoning in humans. Industrial sauce as a matter of day-to-day offer food consumption among the general population have found. This food, suitable for food poisoning. Therefore contamination with Listeria monocytogenes is a threat to health, especially those with suppressed immune systems. The aim of this study is molecular detection of listeria monocytogenes in industrial sauce in Iran. Materials and Methods: 25 Industrial sauces were collected from Iran markets. DNA samples extracted by standard phenol-chloroform method . PCR test optimized and detection of limits (LOD) and specificity evaluated. All of the samples tested with optimized PCR test. Results: PCR test Optimized and 226 bp amplicon was observed in 2% agarose gel electrophoresis. LOD test was obtained 10 genome/reaction and in specificity test, amplicon was not observed with none of microorganism DNA was tested. After PCR testing on 25 sauce samples,4 were positive for Listeria monocytogenes. Conclusion: PCR technique using specific primers very carefully and faster than traditional methods is for the identification of Listeria monocytogenes. The results show that the PCR technique is a good way to monitor microorganisms in food products such as sauces.

Keywords: sauces, detection, Poisoning, Listeria monocytogenes

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