Volume 8, Issue 30 (4-2018)
NCMBJ 2018, 8(30): 9-16 |
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Shabanpishe S, Rezaeian A. The prevalence and frequency of TEM and SHV beta-lactamase resistance genes and CTX-M in Enterobacteriaceae isolated from clinical samples in the laboratory kahooresten. NCMBJ 2018; 8 (30) :9-16
URL: http://ncmbjpiau.ir/article-1-1075-en.html
URL: http://ncmbjpiau.ir/article-1-1075-en.html
Department of Microbiology, Islamic Azad University Jahrom, Jahrom, Iran , rezaeianfon45@gmail.com
Abstract: (5084 Views)
Aim and Background:Today, the presence of broad-spectrum beta-lactamases in bacteria isolated from patients who express multiple drug resistance is a major health problem in most countries.
Materials and Methods:A total of 346 samples of different clinical strains of enterobacteriaceae were isolated from Kahooresten. To determine the resistance of the samples, an antibiotic test was performed. Finally, ESBL isolates were tested by PCR for TEM, CTX-M, and SHV genes.
Results:Of the 346 isolates studied, 162 isolates of Klebsiella and 132 isolates of Escherichia coli were resistant to ceftazidime antibotic or cefotaxime, or both. Also, 82 (42.26%) were isolates producing ESBL.
Conclusions:Due to the high percentage of resistance to third-generation cephalosporins, accurate antibiotic testing before antibiotics is required in infections caused by ESBL-producing organisms.
Materials and Methods:A total of 346 samples of different clinical strains of enterobacteriaceae were isolated from Kahooresten. To determine the resistance of the samples, an antibiotic test was performed. Finally, ESBL isolates were tested by PCR for TEM, CTX-M, and SHV genes.
Results:Of the 346 isolates studied, 162 isolates of Klebsiella and 132 isolates of Escherichia coli were resistant to ceftazidime antibotic or cefotaxime, or both. Also, 82 (42.26%) were isolates producing ESBL.
Conclusions:Due to the high percentage of resistance to third-generation cephalosporins, accurate antibiotic testing before antibiotics is required in infections caused by ESBL-producing organisms.
Type of Study: Research Article |
Subject:
Biochemistry
Received: 2018/04/21 | Accepted: 2018/04/21 | Published: 2018/04/21
Received: 2018/04/21 | Accepted: 2018/04/21 | Published: 2018/04/21
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