Volume 8, Issue 30 (4-2018)
NCMBJ 2018, 8(30): 45-52 |
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Ashkan S A, Zaker Bostanabad S, Mirzaahmadi S. The study of Relationship between miRNA Machinery Genes polymorphism (DROSHA & EXPORTIN5) and recurrent spontaneous abortion in city of tehran
. NCMBJ 2018; 8 (30) :45-52
URL: http://ncmbjpiau.ir/article-1-1076-en.html
URL: http://ncmbjpiau.ir/article-1-1076-en.html
Biology and Microbiology Department, Islamic Azad University, Parand Branch, Tehran, Iran , saeedzaker@piau.ac.ir
Abstract: (15904 Views)
Aim and Background:
Abortion is the most common complication in the first and second trimester of pregnancy. The concept of abortion refers to the termination of pregnancy before the twentieth week of gestation. Women who experience consecutive abortions more than twice can be said to be suffering repeated abortions.
Objective: This study aims at investigating the relationship between the XPO5 rs11077 polymorphism and DROSHA rs10719 miRNA machinery gene in women suffering spontaneous repeated abortions so that it can be used as a diagnostic marker for women with repeated abortions if such a relationship is confirmed.
Materials and Methods: The blood samples of 50 women with spontaneous recurrent abortions and those of 24 women with no history of abortion and at least two successful births were used in this study and the women were selected from those admitted to Masoud labaratory in Tehran. DNA was extracted using a special DNA extraction kit from whole blood samples with EDTA. The XPO5 rs11077 gene polymorphism 2 and DROSHA rs10719 were investigated using the Tetra-ARMS PCR method and analyzed using agarose gel electrophoresis.
Results: The frequencies of Genotypic and allelic rs11077 (A / C) polymorphism of XPO5 gene did not show any significant difference between the two patient and control groups. However, the frequency of genotypic and allelic rs10719 (T / C) polymorphism of DROSHA gene showed a significant difference between the patients and the control samples and TT genotype was shown to be significantly higher in the patient group than in the control group.
Discussion: Our findings suggest that the rs119 (T / C) polymorphism of the DROSHA gene may be associated with the susceptibility to RPL in the Iranian population, and the T allele may increase the incidence of this disease.
Abortion is the most common complication in the first and second trimester of pregnancy. The concept of abortion refers to the termination of pregnancy before the twentieth week of gestation. Women who experience consecutive abortions more than twice can be said to be suffering repeated abortions.
Objective: This study aims at investigating the relationship between the XPO5 rs11077 polymorphism and DROSHA rs10719 miRNA machinery gene in women suffering spontaneous repeated abortions so that it can be used as a diagnostic marker for women with repeated abortions if such a relationship is confirmed.
Materials and Methods: The blood samples of 50 women with spontaneous recurrent abortions and those of 24 women with no history of abortion and at least two successful births were used in this study and the women were selected from those admitted to Masoud labaratory in Tehran. DNA was extracted using a special DNA extraction kit from whole blood samples with EDTA. The XPO5 rs11077 gene polymorphism 2 and DROSHA rs10719 were investigated using the Tetra-ARMS PCR method and analyzed using agarose gel electrophoresis.
Results: The frequencies of Genotypic and allelic rs11077 (A / C) polymorphism of XPO5 gene did not show any significant difference between the two patient and control groups. However, the frequency of genotypic and allelic rs10719 (T / C) polymorphism of DROSHA gene showed a significant difference between the patients and the control samples and TT genotype was shown to be significantly higher in the patient group than in the control group.
Discussion: Our findings suggest that the rs119 (T / C) polymorphism of the DROSHA gene may be associated with the susceptibility to RPL in the Iranian population, and the T allele may increase the incidence of this disease.
Keywords: spontaneous recurrent abortions, machinery genes, DROSHA, XPO5, rs11077 polymorphism, rs10719 polymorphism
Type of Study: Research Article |
Subject:
Microbiology
Received: 2018/04/21 | Accepted: 2018/04/21 | Published: 2018/04/21
Received: 2018/04/21 | Accepted: 2018/04/21 | Published: 2018/04/21
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