Volume 9, Issue 33 (12-2018)
NCMBJ 2018, 9(33): 39-48 |
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Mohamadi M, Jafari M, Heidari M, Eshaghi H, Pournajaf A, Kafshgari R et al . A survey of high-level resistance to gentamicin and aminoglycoside-modifying enzyme-encoding genes in Enterococcus faecalis and Enterococcus faecium isolated from infected burn wounds. NCMBJ 2018; 9 (33) :39-48
URL: http://ncmbjpiau.ir/article-1-1164-en.html
URL: http://ncmbjpiau.ir/article-1-1164-en.html
Mohsen Mohamadi 
, Mohsen Jafari , Morteza Heidari , Hamid Eshaghi , Abazar Pournajaf , Ramin Kafshgari , Mehrdad Gholami
, Mohsen Jafari , Morteza Heidari , Hamid Eshaghi , Abazar Pournajaf , Ramin Kafshgari , Mehrdad Gholami
Abstract: (5038 Views)
Aim and Background: The aim of this study was to survey of High-level resistance to gentamicin and aminoglycoside-modifying enzymes (AMEs) encoding genes in Enterococcus faecalis and Enterococcus faecium isolated from burn wound infection.
Material and methods: Antibiotic susceptibility test was performed after identification of E. faecalis and E. faecium strains. In order to detect HLGR strains, gentamicin disks were used. PCR test was performed to identify the AMEs-encoding genes.
Results: Of 114 isolates of Enterococcus, 56.1% of the strains were HLGR. All HLGR strains have the aac (6') -Ie -aph (2'') -Ia gene. 1.9% of the HLGR E. faecium strains carried the aph (2 ") -Id gene.
Conclusions: Our data showed that a high prevalence of resistance to gentamicin in burned wound infection in our hospital. The main responsibility for the resistance to gentamicin in the present study was the aac (6 ') -Ie -aph (2'') -Ia gene.
Material and methods: Antibiotic susceptibility test was performed after identification of E. faecalis and E. faecium strains. In order to detect HLGR strains, gentamicin disks were used. PCR test was performed to identify the AMEs-encoding genes.
Results: Of 114 isolates of Enterococcus, 56.1% of the strains were HLGR. All HLGR strains have the aac (6') -Ie -aph (2'') -Ia gene. 1.9% of the HLGR E. faecium strains carried the aph (2 ") -Id gene.
Conclusions: Our data showed that a high prevalence of resistance to gentamicin in burned wound infection in our hospital. The main responsibility for the resistance to gentamicin in the present study was the aac (6 ') -Ie -aph (2'') -Ia gene.
Keywords: Gentamicin, Aminoglycoside modifying enzymes, Enterococcus faecalis, Enterococcus faecium, Burn wound.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2018/12/22 | Accepted: 2018/12/22 | Published: 2018/12/22
Received: 2018/12/22 | Accepted: 2018/12/22 | Published: 2018/12/22
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