Volume 9, Issue 36 (9-2019)                   NCMBJ 2019, 9(36): 67-76 | Back to browse issues page

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khoshbin M, shahhosseiny M H, Paknejadi M. Molecular diagnosis of Helicobacter pylori in semen of infertile men. NCMBJ. 2019; 9 (36) :67-76
URL: http://ncmbjpiau.ir/article-1-1216-en.html
Department of Microbiology, Basic Sciences, shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
Abstract:   (2550 Views)
Aim and Background: Bacterial infections of the genital system are common causes of infertility. One of the most important causes of male infertility is seminal and genital tract infections. In the meantime, a wide range of bacteria, in varying degrees, contribute to infertility in men. Helicobacter pylori may be involve in infertility. Because antibodies against this bacteria have been significantly detected in the genital fluids of infertile patients. The aim of this study was to determine the presence of Helicobacter pylori in the semen of the infertile men with rapid molecular PCR method and determine its percentage.
Materials and Methods: 100 samples of semen collected from Saram hospital and DNA Extracted from these specimens using Boiling/DNG_PLUS method.  Optimized PCR test was performed on samples. PCR test evaluated from the respect sensitivity and specificity.
Results: In this study, amplicon with the size of 294 bp, observed with agarose electrophoresis.  In the specificity test, primers created only a band for H.pylori DNA. Of the 100 samples tested, only 10 samples (10%) were positive for DNA of H.pylori.
Conclusion: According to the results of this study, H.pylori may be one of the bacterial agents that can be considered for male infertility, of course requires further studies. While PCR is suitable, quick and sensitive molecular technique for detection of H.pylori in infertile men.
 
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Type of Study: Research Article | Subject: Cellular and molecular
Received: 2019/08/26 | Accepted: 2019/08/26 | Published: 2019/08/26

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