Volume 9, Issue 36 (9-2019)
NCMBJ 2019, 9(36): 77-88 |
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Moradi Z, Zaker Bostanabad S, Hadighi R. Study of Leishmania Tropica proliferation in mouse J774A-1 cell line.. NCMBJ 2019; 9 (36) :77-88
URL: http://ncmbjpiau.ir/article-1-1217-en.html
URL: http://ncmbjpiau.ir/article-1-1217-en.html
Department of Parasitology, Faculty of Public Health, Iran University of Medical Sciences, Tehran, Iran,
Abstract: (4006 Views)
Aim and Background:
From the perspective of biology The genus Leishmania belongs to Tripanozomatite tribe flagellate Parasits. Leishmania is in the form of promastigotes and amastigotes. In culture, In the form of promastigotes and intra-macrophage is in the form of amastigotes. The aim of this study was to investigate the survival of L.tropica parasites in culture .
Materials and Methods:
In this study, the Possibility of growth in NNN and RPMI1640 culture Reviewed . In the laboratory, Conditions were provided that L.tropica protighotites transformed into amastigotes that live in the host cell. Growth of L.tropica was investigated in comparison with other types of leishmaniasis, such as L. major. L.tropica was kept at 25-26 ° C in a conventional incubator. L.tropica culture culture was performed in RPMI1640 culture. Contamination of L.tropica was done on J774A-1 cells. The J774A-1 cell was maintained in RPMI1640 medium. Cells were stored at 37 ° C in a CO2-encoded incubator.
Results:
L.tropica After infecting was observed on the J774A-1 macrophage cell line. compared to L.major, Which takes more time to show its performance, L.tropica is the power of survival for a long time Inside it . It was also observed that L.tropica was only stored and not proliferated in NNN medium.
Conclusion:
The results indicate that L.tropica protighotites, in laboratory environment Became amastigotes Which host cells have the power of survival for a long time.
From the perspective of biology The genus Leishmania belongs to Tripanozomatite tribe flagellate Parasits. Leishmania is in the form of promastigotes and amastigotes. In culture, In the form of promastigotes and intra-macrophage is in the form of amastigotes. The aim of this study was to investigate the survival of L.tropica parasites in culture .
Materials and Methods:
In this study, the Possibility of growth in NNN and RPMI1640 culture Reviewed . In the laboratory, Conditions were provided that L.tropica protighotites transformed into amastigotes that live in the host cell. Growth of L.tropica was investigated in comparison with other types of leishmaniasis, such as L. major. L.tropica was kept at 25-26 ° C in a conventional incubator. L.tropica culture culture was performed in RPMI1640 culture. Contamination of L.tropica was done on J774A-1 cells. The J774A-1 cell was maintained in RPMI1640 medium. Cells were stored at 37 ° C in a CO2-encoded incubator.
Results:
L.tropica After infecting was observed on the J774A-1 macrophage cell line. compared to L.major, Which takes more time to show its performance, L.tropica is the power of survival for a long time Inside it . It was also observed that L.tropica was only stored and not proliferated in NNN medium.
Conclusion:
The results indicate that L.tropica protighotites, in laboratory environment Became amastigotes Which host cells have the power of survival for a long time.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2019/08/27 | Accepted: 2019/08/27 | Published: 2019/08/27
Received: 2019/08/27 | Accepted: 2019/08/27 | Published: 2019/08/27
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