Volume 10, Issue 37 (12-2019)
NCMBJ 2019, 10(37): 109-120 |
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Hoseini M, Sazgar H, Zia Jahromi N. Study of the frequencies of rs137852579 in Androgen receptor coding gene in patients suffering from prostate cancer and theoretical study of the association of this polymorphism with drug resistance to treatment with enzalutamide. NCMBJ 2019; 10 (37) :109-120
URL: http://ncmbjpiau.ir/article-1-1247-en.html
URL: http://ncmbjpiau.ir/article-1-1247-en.html
Department of Biology, Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Abstract: (3395 Views)
Aim and Background: Many single nucleotide polymorphisms have been identified in the androgen receptor coding gene that play an important role in the development, prediction, and diagnosis of cancer. In this regard, this study was designed to evaluate the allelic frequency of rs137852579 single-nucleotide polymorphism in the androgen receptor coding gene in patients suffering from prostate cancer.
Material and methods: In this study, peripheral blood was taken in 10cc out of 50 prostate cancer patients and 50 healthy individuals. Genomic DNA of the samples was extracted and isolated. ARMS-PCR and direct sequencing were used to determine the presence of the desired polymorphism in population.
Results: The results of this study showed that the allele frequency of rs137852579 for the C allele in the healthy group was 0.06 and in the patient group was 0.28. In addition, the heterozygosity rate showed that the Iranian population was in Hardy Weinberg equilibrium for this polymorphism (p> 0.05).
Conclusion: Based on the results of this study, it can be concluded that rs137852579 polymorphism in the androgen receptor gene can contribute to prostate cancer in the Iranian population.
Material and methods: In this study, peripheral blood was taken in 10cc out of 50 prostate cancer patients and 50 healthy individuals. Genomic DNA of the samples was extracted and isolated. ARMS-PCR and direct sequencing were used to determine the presence of the desired polymorphism in population.
Results: The results of this study showed that the allele frequency of rs137852579 for the C allele in the healthy group was 0.06 and in the patient group was 0.28. In addition, the heterozygosity rate showed that the Iranian population was in Hardy Weinberg equilibrium for this polymorphism (p> 0.05).
Conclusion: Based on the results of this study, it can be concluded that rs137852579 polymorphism in the androgen receptor gene can contribute to prostate cancer in the Iranian population.
Keywords: Drug Resistance, Prostate Cancer, Single-Nucleotide Polymorphism, Bioinformatics Analysis.IAU-science
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2019/12/5 | Accepted: 2019/12/5 | Published: 2019/12/5
Received: 2019/12/5 | Accepted: 2019/12/5 | Published: 2019/12/5
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