Volume 10, Issue 37 (12-2019)
NCMBJ 2019, 10(37): 89-108 |
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Dourr S, Dehghani Bidgoli R, Akhbari M, Razmjoue D. Evaluation and Comparison of Phenolic Compounds and Antioxidant Activity Extract of Several populationof Narcissus sp. From Behbahan city. NCMBJ 2019; 10 (37) :89-108
URL: http://ncmbjpiau.ir/article-1-1251-en.html
URL: http://ncmbjpiau.ir/article-1-1251-en.html
Department of Chemistry, Natural Essential Oils Institute, University of Kashan, Kashan, Iran
Abstract: (3626 Views)
Aim and Background:Narcissus family plants are important in addition to their ornamental value in terms of aromatic and medicinal compounds. The present study was conducted to investigate the quantitative and qualitative properties of phenolic compounds and antioxidant activity of four genotypes of Narcissus with the local name: Shahla, Meskin, Shasteper and Panjeh gorbeie in two phenological stages (beginning and end of harvest season).
Material and methods:For this purpose, after collecting plant materials in the natural habitats of Behbahan, drying and cold extraction were done. In this experimental study, the phytochemical study of the plant was first performed, then the total phenolic and flavonoid compounds were measured by spectrophotometric method. Finally, the antioxidant activity of the extract at different concentrations was determined using DPPH assay.
Results:The results showed that the extract yield in the sample of Maskin was more than the other samples at the beginning of harvest season. Also, compounds such as tannin and alkaloids were observed in some samples, that, varied in different these genotypes. The high percentage of DPPH inhibition is also confirmed the high antioxidant properties of these genotypes, although the three genotypes had the same degree of DPPH inhibition level, but the highest antioxidant activity is related to Meshkin with inhibitory percent 72.93 and the least anti-oxidant property related to Shahla with an inhibition rate of 91.895. According to the results, Maskin and Shahla genotypes showed the highest and least antioxidant properties, respectively.
Conclusion:The results of this study indicate that there is a significant difference in the composition and percentage of inhibition of DPPH free radicals in the extract of various nursery plants, which has not been reported for this plant in scientific sources.
Material and methods:For this purpose, after collecting plant materials in the natural habitats of Behbahan, drying and cold extraction were done. In this experimental study, the phytochemical study of the plant was first performed, then the total phenolic and flavonoid compounds were measured by spectrophotometric method. Finally, the antioxidant activity of the extract at different concentrations was determined using DPPH assay.
Results:The results showed that the extract yield in the sample of Maskin was more than the other samples at the beginning of harvest season. Also, compounds such as tannin and alkaloids were observed in some samples, that, varied in different these genotypes. The high percentage of DPPH inhibition is also confirmed the high antioxidant properties of these genotypes, although the three genotypes had the same degree of DPPH inhibition level, but the highest antioxidant activity is related to Meshkin with inhibitory percent 72.93 and the least anti-oxidant property related to Shahla with an inhibition rate of 91.895. According to the results, Maskin and Shahla genotypes showed the highest and least antioxidant properties, respectively.
Conclusion:The results of this study indicate that there is a significant difference in the composition and percentage of inhibition of DPPH free radicals in the extract of various nursery plants, which has not been reported for this plant in scientific sources.
Keywords: Phytochemical test, Antioxidant properties, Inhibitory percentage, Narcissus, Extract, IAU-science
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2019/12/5 | Accepted: 2019/12/5 | Published: 2019/12/5
Received: 2019/12/5 | Accepted: 2019/12/5 | Published: 2019/12/5
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