Volume 10, Issue 37 (12-2019)                   NCMBJ 2019, 10(37): 35-52 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jameee N, Hosseini S S, Akrami H R, Dehghani Ashkzari M. Evaluation of cytotoxicity effect of Synthetic Pyridopyrimidine derivatives on the cancer vs. normal cell lines. NCMBJ. 2019; 10 (37) :35-52
URL: http://ncmbjpiau.ir/article-1-1252-en.html
Medical Biotechnology Research Center, Ashekdarz, Yazd, Iran
Abstract:   (2691 Views)
Aim and Background: Recently, studies have been reported in vitro and in vivo, that these pyridopyrimidine compounds have a beneficial effect on chronic diseases such as cancer. this study was designed to evaluate the effects of synthetic pyridopyrimidine compounds on the growth and proliferation of normal and cancer cell lines.
Material and Methods: Cytotoxic effect of synthetic pyridopyrimidine at different concentrations and period on the MCF-7, MAD-MB-231, MOLT-4, PC-3, and Normal Fibroblast (HFF) cell lines were cultured in 96-well plates, thin cells were treated with synthetic compounds at different concentrations 1000, 500, 50, 5 nM at 24,48 and 72 hours, to evaluate the effect using MTT assay. the resulting data were analyzed using SPSS16 software with one-way ANOVA followed by the Tukey test and P <0.05 was considered as a significant difference.
Results: The results of this study showed that pyridopyrimidine compounds had no significant toxicity effect on the normal cell lines, Nevertheless showed strong and dose-dependent inhibition of cancer cell growth at P-value <0.05. also, results showed that pyridopyrimidines with bromide groups at a concentration of 500 to 1000 nM had a more effective effect on inhibition of cancer cell growth inhibition at 72 hours.
Conclusion: Results of this study indicated the pyrimidines inhibition effect on cancer cell lines, however, the use of these compounds as an attractive option in chronic diseases treatment in the world as alternative medicines or combination with conventional medicines are suggested
Keywords: Pyridopyrimidines,Normal and Cancer Cell lines, MTT assay, IC50Aim and Background: Recently, studies have been reported in vitro and in vivo, that these pyridopyrimidine compounds have a beneficial effect on chronic diseases such as cancer. this study was designed to evaluate the effects of synthetic pyridopyrimidine compounds on the growth and proliferation of normal and cancer cell lines.
Material and Methods: Cytotoxic effect of synthetic pyridopyrimidine at different concentrations and period on the MCF-7, MAD-MB-231, MOLT-4, PC-3, and Normal Fibroblast (HFF) cell lines were cultured in 96-well plates, thin cells were treated with synthetic compounds at different concentrations 1000, 500, 50, 5 nM at 24,48 and 72 hours, to evaluate the effect using MTT assay. the resulting data were analyzed using SPSS16 software with one-way ANOVA followed by the Tukey test and P <0.05 was considered as a significant difference.
Results: The results of this study showed that pyridopyrimidine compounds had no significant toxicity effect on the normal cell lines, Nevertheless showed strong and dose-dependent inhibition of cancer cell growth at P-value <0.05. also, results showed that pyridopyrimidines with bromide groups at a concentration of 500 to 1000 nM had a more effective effect on inhibition of cancer cell growth inhibition at 72 hours.
Conclusion: Results of this study indicated the pyrimidines inhibition effect on cancer cell lines, however, the use of these compounds as an attractive option in chronic diseases treatment in the world as alternative medicines or combination with conventional medicines are suggested
 
Full-Text [PDF 891 kb]   (825 Downloads)    
Type of Study: Research Article | Subject: Cellular and molecular
Received: 2019/12/5 | Accepted: 2019/12/5 | Published: 2019/12/5

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2021 CC BY-NC 4.0 | New Cellular and Molecular Biotechnology Journal

Designed & Developed by : Yektaweb