Volume 11, Issue 44 (9-2021)
NCMBJ 2021, 11(44): 9-18 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Babajani ahmad saraei S, Shahhosseiny M H, Aliasgari E. PCR Detection of Herpes Simplex Virus 2 in Idiopathic Abortions. NCMBJ 2021; 11 (44) :9-18
URL: http://ncmbjpiau.ir/article-1-1415-en.html
URL: http://ncmbjpiau.ir/article-1-1415-en.html
Department of biology, East Tehran Branch, Islamic Azad University,Tehran,Iran
Abstract: (2264 Views)
Abstract
Aims and Background: Herpes simplex virus type 2 (HSV-2) is one of the most important human pathogenic viruses. The virus causes genital herpes and can cause miscarriage, pregnancy complications, and infertility in women. The aim of this study was to evaluate the role of HSV-2 in idiopathic abortion by PCR.
Materials and Methods: Fifty blood serum samples of women with idiopathic miscarriages were collected and transferred to the laboratory. The PCR test was performed using standard HSV-2 strain DNA and then the limit of detection (LOD) and specificity of the test was determined using a different strain of DNA. A standard test was performed using positive and negative controls on the samples and the results were analyzed by agarose gel electrophoresis.
Results: The PCR technique est optimized and 231 bp amplicon were observed in agarose gel. In specificity test only with DNA HSV-2 achieved positive results as well as a limit of detection 10 Copy / Reaction. Of the 50 samples studied, 9 (4.5%) were positive.
Conclusion: Idiopathic miscarriages are miscarriages that do not seem to have a definite cause. But clearly, several factors, including infectious agents that are difficult to identify, can cause miscarriage. One of these factors could be HSV-2. The results of this study showed that HSV-2 is one of the most important factors in this type of miscarriage and that HSV-2 can be quickly identified by tests such as PCR.
Aims and Background: Herpes simplex virus type 2 (HSV-2) is one of the most important human pathogenic viruses. The virus causes genital herpes and can cause miscarriage, pregnancy complications, and infertility in women. The aim of this study was to evaluate the role of HSV-2 in idiopathic abortion by PCR.
Materials and Methods: Fifty blood serum samples of women with idiopathic miscarriages were collected and transferred to the laboratory. The PCR test was performed using standard HSV-2 strain DNA and then the limit of detection (LOD) and specificity of the test was determined using a different strain of DNA. A standard test was performed using positive and negative controls on the samples and the results were analyzed by agarose gel electrophoresis.
Results: The PCR technique est optimized and 231 bp amplicon were observed in agarose gel. In specificity test only with DNA HSV-2 achieved positive results as well as a limit of detection 10 Copy / Reaction. Of the 50 samples studied, 9 (4.5%) were positive.
Conclusion: Idiopathic miscarriages are miscarriages that do not seem to have a definite cause. But clearly, several factors, including infectious agents that are difficult to identify, can cause miscarriage. One of these factors could be HSV-2. The results of this study showed that HSV-2 is one of the most important factors in this type of miscarriage and that HSV-2 can be quickly identified by tests such as PCR.
Keywords: Herpes simplex virus 2, polymerase chain reaction, diagnosis, idiopathic abortion, Iau Science .
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2020/09/12 | Accepted: 2021/08/9 | Published: 2021/09/1
Received: 2020/09/12 | Accepted: 2021/08/9 | Published: 2021/09/1
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
