Volume 12, Issue 48 (10-2022)
NCMBJ 2022, 12(48): 87-98 |
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Bonyadi P, Shojaee saadi B, Amini K. Evaluation of the effect of ionic liquids based on amino acid on the expression of pathogenic genes of Enterococcus faecalis strains isolated from the mouth. NCMBJ 2022; 12 (48) : 7
URL: http://ncmbjpiau.ir/article-1-1430-en.html
URL: http://ncmbjpiau.ir/article-1-1430-en.html
Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran , dr_kumarss_amini@yahoo.com
Abstract: (1052 Views)
Aim and Background: Enterococcus faecalis species are significant issue in severe nosocomial infections. In this study we aimed to investigate the effect of ionic liquids based on amino acids on pathogenic genes of this bacterium using Real time PCR.
Material and Methods: In this study, 100 samples of oral infections were taken from patients and Enterococcus faecalis strains were identified using biochemical tests. The presence and frequency of cyl, hyl and esp genes and their expression under the influence of ionic fluid were evaluated using real time-PCR.
Results: From the total samples taken, twelve Enterococcus faecalis bacteria were isolated and identified. The highest frequency of pathogenic genes was related to cyl gene which was present in 11 strains and the expression of target genes in the treated group under the influence of ionic fluid decreased compared to the control group (p <0.05). With decreasing ion-based amino acid concentration, the ability of bacteria to form biofilms increased (p <0.05).
Conclusion: Widespread antibiotic resistance is the most important concern about Enterococcus and cytolysin is a gelatinase enzyme and one of the most important invasive agents of Enterococcus faecalis. The results of this study provide a basis for promoting the use of new antibacterial agents, including ionic fluid, to prevent further pathogenicity of Enterococcus faecalis.
Material and Methods: In this study, 100 samples of oral infections were taken from patients and Enterococcus faecalis strains were identified using biochemical tests. The presence and frequency of cyl, hyl and esp genes and their expression under the influence of ionic fluid were evaluated using real time-PCR.
Results: From the total samples taken, twelve Enterococcus faecalis bacteria were isolated and identified. The highest frequency of pathogenic genes was related to cyl gene which was present in 11 strains and the expression of target genes in the treated group under the influence of ionic fluid decreased compared to the control group (p <0.05). With decreasing ion-based amino acid concentration, the ability of bacteria to form biofilms increased (p <0.05).
Conclusion: Widespread antibiotic resistance is the most important concern about Enterococcus and cytolysin is a gelatinase enzyme and one of the most important invasive agents of Enterococcus faecalis. The results of this study provide a basis for promoting the use of new antibacterial agents, including ionic fluid, to prevent further pathogenicity of Enterococcus faecalis.
Article number: 7
Type of Study: Research Article |
Subject:
Microbiology
Received: 2021/11/7 | Accepted: 2022/02/27 | Published: 2022/10/23
Received: 2021/11/7 | Accepted: 2022/02/27 | Published: 2022/10/23
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