Volume 13, Issue 49 (12-2022)
NCMBJ 2022, 13(49): 35-44 |
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Ahsani Arani Y, Noormohammadi Z, Rasekh B, Yazdian F, Kazemi H. Evaluation of rhamnosyl transferase enzyme expression in the bio surfactant production pathway of Pseudomonas aeruginosa bacteria in the presence of SDS-based iron nanostructure. NCMBJ 2022; 13 (49) :35-44
URL: http://ncmbjpiau.ir/article-1-1474-en.html
URL: http://ncmbjpiau.ir/article-1-1474-en.html
Director Environment & Biotechnology Research Division, Research Institute of Petroleum Industry (RIPI), Tehran, Iran , rasekhb@ripi.ir
Abstract: (946 Views)
Aim and Background: Rhamnolipids are one of the most important bio surfactants of glycolipids produced by Pseudomonas aeruginosa. In this study, the expression of bio surfactant producing proteins of Pseudomonas aeruginosa in the presence of sodium dodecyl sulfate coated iron nanostructure (Fe/SDS) were evaluated.
Materials and Methods: The expression of Surfactant Protein A enzyme was investigated by SDS-PAGE and Western blot. The enzyme rhamnosyl transferase was analyzed on SDS-PAGE gel and confirmed by Western blotting. The presence of nanoparticles on the surface of bacteria and the morphology of nanoparticles were investigated by TEM and SEM.
Results: According to SEM, nanoparticles with a size of 20 nm and a spherical shape were seen. Binding of Fe / SDS nanoparticles to bio surfactant was confirmed by TEM. The enzyme rhamnosyl transferase with a molecular weight of 40 kDa was expressed in the test sample. The results of protein expression showed that the confirmed of rhamnosyl transferase enzyme increased significantly under the influence of Fe/SDS nanoparticles and showed the confirmed compared to other nanoparticles and the control sample. While SDS and Fe nanoparticles alone are not effective in confirmed this protein and are almost identical to the control sample. Western blotting results of SDS-PAGE confirmed the expression of rhamnolipid transferase under the influence of Fe/SDS nanoparticles.
Conclusion: The results of the present study indicate the importance of Fe/SDS nanoparticles in increasing the expression of rhamnolipid transferase enzyme in the production of bio surfactants from Pseudomonas aeruginosa.
Materials and Methods: The expression of Surfactant Protein A enzyme was investigated by SDS-PAGE and Western blot. The enzyme rhamnosyl transferase was analyzed on SDS-PAGE gel and confirmed by Western blotting. The presence of nanoparticles on the surface of bacteria and the morphology of nanoparticles were investigated by TEM and SEM.
Results: According to SEM, nanoparticles with a size of 20 nm and a spherical shape were seen. Binding of Fe / SDS nanoparticles to bio surfactant was confirmed by TEM. The enzyme rhamnosyl transferase with a molecular weight of 40 kDa was expressed in the test sample. The results of protein expression showed that the confirmed of rhamnosyl transferase enzyme increased significantly under the influence of Fe/SDS nanoparticles and showed the confirmed compared to other nanoparticles and the control sample. While SDS and Fe nanoparticles alone are not effective in confirmed this protein and are almost identical to the control sample. Western blotting results of SDS-PAGE confirmed the expression of rhamnolipid transferase under the influence of Fe/SDS nanoparticles.
Conclusion: The results of the present study indicate the importance of Fe/SDS nanoparticles in increasing the expression of rhamnolipid transferase enzyme in the production of bio surfactants from Pseudomonas aeruginosa.
Keywords: Bio surfactant, Fe/SDS nanoparticle, Pseudomonas aeruginosa, Western blot, SDS-PAGE, Iau Science
Type of Study: Research Article |
Subject:
Microbiology
Received: 2022/02/16 | Accepted: 2022/03/17 | Published: 2023/01/3
Received: 2022/02/16 | Accepted: 2022/03/17 | Published: 2023/01/3
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