Volume 12, Issue 47 (7-2022)
NCMBJ 2022, 12(47): 23-32 |
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Jamshidi Moghaddam E, Zaker Bostan Abad S, Rahimi M K. Genotyping of Brucella bacteria isolated from samples of patients suspected of brucellosis by technique PCR-RFLP in Tehran(2017-2020). NCMBJ 2022; 12 (47) :23-32
URL: http://ncmbjpiau.ir/article-1-1477-en.html
URL: http://ncmbjpiau.ir/article-1-1477-en.html
Department of Microbiology, Faculty Medicine, Tehran medical science, Islamic Azad university,Tehran,Iran
Abstract: (1320 Views)
Aim and Background: Brucellosis is one of the most important diseases of zoonosis and is very important in many countries, including Iran, from a health and economic point of view. In this study, the Genotyping of Brucella bacteria isolated from samples of patients suspected of brucellosis was evaluated by PCR RFLP in Tehran.
Material and methods: Out of 453 Blood samples suspected of brucellosis of patients who had referred to laboratories in Tehran were inoculated in Castanida culture media and stored at 37 ° C for 21 days. DNA was extracted from colonies that had grown on the surface. Brucella omp2a gene was amplified by PCR using a specific primer. PCR products were analyzed with Pst1 restriction enzyme, digestion and RFLP patterns.
Results: Out of the total samples, it grew in 10 colonies and was confirmed by Brucella by PCR method. In RFLP test, in 8 specimens, Brucella melitensis and in 2 specimens, Brucella abortus were detected.
Conclusion:There is brucellosis in our country,infections are also observed in some villages,and among these cases, Brucella melitensis is more common than other species and with the help of molecular methods, in the shortest possible time, high accuracy and sensitivity and with less risk, the presence of different diseases and species of Brucella can be detected.
Material and methods: Out of 453 Blood samples suspected of brucellosis of patients who had referred to laboratories in Tehran were inoculated in Castanida culture media and stored at 37 ° C for 21 days. DNA was extracted from colonies that had grown on the surface. Brucella omp2a gene was amplified by PCR using a specific primer. PCR products were analyzed with Pst1 restriction enzyme, digestion and RFLP patterns.
Results: Out of the total samples, it grew in 10 colonies and was confirmed by Brucella by PCR method. In RFLP test, in 8 specimens, Brucella melitensis and in 2 specimens, Brucella abortus were detected.
Conclusion:There is brucellosis in our country,infections are also observed in some villages,and among these cases, Brucella melitensis is more common than other species and with the help of molecular methods, in the shortest possible time, high accuracy and sensitivity and with less risk, the presence of different diseases and species of Brucella can be detected.
Type of Study: Research Article |
Subject:
Physiology
Received: 2021/11/8 | Accepted: 2022/01/4 | Published: 2022/04/19
Received: 2021/11/8 | Accepted: 2022/01/4 | Published: 2022/04/19
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