Volume 13, Issue 49 (12-2022)
NCMBJ 2022, 13(49): 45-54 |
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Reamasouleh F, Arasteh A, Ataei-e Jaliseh S. phytochemical evaluation, antimicrobial and inhibitory effects of Ginger extract on amyloid Nano–fibril production in order to evaluate its application potential against inflammatory and Alzheimer's disease. NCMBJ 2022; 13 (49) :45-54
URL: http://ncmbjpiau.ir/article-1-1533-en.html
URL: http://ncmbjpiau.ir/article-1-1533-en.html
Department of biology, Rasht Branch, Islamic Azad University, Rasht, Iran
Abstract: (945 Views)
Aim and Background: Ginger with the scientific name of Zingiber officinale contains the active ingredient Gingerol and many antioxidant and antimicrobial compounds. The aim of this study was to investigate the antimicrobial properties of aqueous and hydro–alcoholic extracts of ginger and to investigate the anti-Alzheimer's effect of its hydro–alcoholic extract by inhibiting the production of amyloid nanofibers.
Materials and Methods: After milling, aqueous and hydro–alcoholic extract of ginger was prepared by soaking method and its antimicrobial effects on Escherichia coli and Staphylococcus aureus were investigated using agar diffusion method and determination of MIC and MBC. Visible spectroscopy was used to determine the inhibitory effect of hydro–alcoholic extract on the production of amyloid filaments.
Results: The results showed that the diameter of the growth inhibition zone due to hydro–alcoholic extract of ginger in the well method was 15 mm for Staphylococcus aureus and 10 mm for Escherichia coli. The MIC and MBC levels for both bacteria were 50 and 100 mg / ml, respectively, and the aqueous extract of ginger had no effect on Escherichia coli. Increasing the concentration of ginger extract in the sample reduced the production of amyloid fibrils, which was associated with decreased absorption and redshift in the visible absorption spectrum of the Congored. The presence of the active ingredient gingerol was confirmed by GC–Mass method up to 34.7%.
Conclusion: Ginger extract with the presence of antimicrobial compounds such as gingerol, with good performance and lower side effects and cost, can be introduced as an effective medicinal plant for reducing the effects of inflammatory and Alzheimer's disease.
Materials and Methods: After milling, aqueous and hydro–alcoholic extract of ginger was prepared by soaking method and its antimicrobial effects on Escherichia coli and Staphylococcus aureus were investigated using agar diffusion method and determination of MIC and MBC. Visible spectroscopy was used to determine the inhibitory effect of hydro–alcoholic extract on the production of amyloid filaments.
Results: The results showed that the diameter of the growth inhibition zone due to hydro–alcoholic extract of ginger in the well method was 15 mm for Staphylococcus aureus and 10 mm for Escherichia coli. The MIC and MBC levels for both bacteria were 50 and 100 mg / ml, respectively, and the aqueous extract of ginger had no effect on Escherichia coli. Increasing the concentration of ginger extract in the sample reduced the production of amyloid fibrils, which was associated with decreased absorption and redshift in the visible absorption spectrum of the Congored. The presence of the active ingredient gingerol was confirmed by GC–Mass method up to 34.7%.
Conclusion: Ginger extract with the presence of antimicrobial compounds such as gingerol, with good performance and lower side effects and cost, can be introduced as an effective medicinal plant for reducing the effects of inflammatory and Alzheimer's disease.
Type of Study: Research Article |
Subject:
Physiology
Received: 2022/01/20 | Accepted: 2022/03/30 | Published: 2022/12/22
Received: 2022/01/20 | Accepted: 2022/03/30 | Published: 2022/12/22
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