Volume 13, Issue 52 (9-2023)                   NCMBJ 2023, 13(52): 9-22 | Back to browse issues page

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Mosavikani E, Noor E, Bandehpour M, Mostafazadeh A, YousefGhahari B, Majidi F, et al . System biology analysis of Rheumatoid Arthritis and evaluation of the IL-6 and IL-6R genes expression in treated patients. NCMBJ 2023; 13 (52) :9-22
URL: http://ncmbjpiau.ir/article-1-1623-en.html
1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Behesht1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2.2. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran i University of Medical Sciences, Tehran, Iran , m.Bandehpour@sbmu.ac.ir
Abstract:   (377 Views)
Aim and Background: Rheumatoid arthritis(RA) is a complex and chronic disease of joint inflammation. Environmental factors that are important in genetically predisposed people are involved in the occurrence of the disease and its progress. This disease has a lot to related to genetics, including human class 2 antigens. In various studies, this relation is about 60%. This relationship is more positive in people with Anti-citrullinated protein antibodies (ACPA) than negative ones. The aim of this study is to investigate the relationship between cytokines and soluble proteins in the body of a patient with Rheumatoid arthritis before symptoms appear.
Materials and Methods: In this research, analysis with Enrichr, WebGestalt, and GeneMANIA was used to find genes related to the disease. In order to check the amount of cytokines predicted, ELISA and Real time RT-PCR methods were applied. Results: By examining the System Biology methods, the candidate genes included IL-6R, IL-6 and IL-23A. The samples were taken from 46 RA patients referred to the rheumatology department of the Babol hospital, and a significant decrease (Pv=0.1255) of IL-6 was observed in patients undergoing treatment and a significant increase (Pv=0.4414) in the amount of this cytokine receptor in the serum compared to healthy individuals. While the expression of IL6 and IL6R genes increased in the patient group compared to the healthy group.
Conclusion: Cytokine IL-6 as an inflammatory cytokine in treated patients decreased in serum and showed a significant increase in RNA level.


 
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Type of Study: Research Article | Subject: Physiology
Received: 2023/12/2 | Accepted: 2023/09/23 | Published: 2023/09/23

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