Volume 14, Issue 54 (5-2024)
NCMBJ 2024, 14(54): 43-52 |
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Naseh V, Salehypour M, Moghadas A, Farzaneh S. Determining the effective dose of cadmium on viability and connexin43 gene expression in RAW264-7 cell line. NCMBJ 2024; 14 (54) :43-52
URL: http://ncmbjpiau.ir/article-1-1657-en.html
URL: http://ncmbjpiau.ir/article-1-1657-en.html
Department of Biology, Parand Branch, Islamic Azad university, Tehran, Iran. , Vahid55vet@yahoo.com
Abstract: (942 Views)
Aim and Background: Toxic metals such as cadmium and the oxidative stress caused by them can develop many Disease such as atherosclerosis. Atherosclerosis is a progressive chronic inflammatory disease that leads to the coronary ischemic with the accumulation of fats at the branching point of the vessels. Dysfunction of many genes, such as connexin, which play a role in lipid homeostasis, can cause atherosclerosis. The present study investigates the effect of cadmium on the expression level of connexin 43 gene in RAW264-7 cell line.
Material and methods: In this study, RAW264-7 cell line was cultured and affected by different doses of cadmium chloride. IC50 was determined using MTT test, RNA was extracted and connexin 43 gene expression was evaluated by Real Time PCR method. The results were analyzed using SPSS software with oneway ANOVA statistical method.
Result: Our findings showed that the expression of connexin 43 gene in polluted cells by 6.3 μM cadmium had a significant increase compared with control group, especially in a short time. Also, the IC50 results indicate a decrease in cell viability in this concentration.
Conclusion: It seems that cadmium in moderate concentrations and in a short time can exert cytotoxic effects on the scavenger receptors of macrophage cells by increasing the expression of connexin 43 gene in RAW264-7 cell line and lead to the development of atherosclerosis.
Material and methods: In this study, RAW264-7 cell line was cultured and affected by different doses of cadmium chloride. IC50 was determined using MTT test, RNA was extracted and connexin 43 gene expression was evaluated by Real Time PCR method. The results were analyzed using SPSS software with oneway ANOVA statistical method.
Result: Our findings showed that the expression of connexin 43 gene in polluted cells by 6.3 μM cadmium had a significant increase compared with control group, especially in a short time. Also, the IC50 results indicate a decrease in cell viability in this concentration.
Conclusion: It seems that cadmium in moderate concentrations and in a short time can exert cytotoxic effects on the scavenger receptors of macrophage cells by increasing the expression of connexin 43 gene in RAW264-7 cell line and lead to the development of atherosclerosis.
Type of Study: Research Article |
Subject:
Physiology
Received: 2024/05/14 | Accepted: 2024/05/12 | Published: 2024/05/12
Received: 2024/05/14 | Accepted: 2024/05/12 | Published: 2024/05/12
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