Volume 14, Issue 54 (5-2024)
NCMBJ 2024, 14(54): 87-96 |
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Salman Hormati A, Yari R, Mirzaee M. Application of multiplex-PCR in oxa and ndm genes assessment and definition of antibiotic resistance pattern in Acinetobacter baumannii clinical samples. NCMBJ 2024; 14 (54) :87-96
URL: http://ncmbjpiau.ir/article-1-1660-en.html
URL: http://ncmbjpiau.ir/article-1-1660-en.html
Department of Biology, Medicinal Plants, Health and Food Security Research Center, Borujerd Branch, Islamic Azad University, Borujerd, Iran , rezayari@yahoo.com
Abstract: (635 Views)
Aim and Background: Acinetobacter baumannii is the cause of a wide range of nosocomial infections. Antibiotic resistance of this organism is a major challenge worldwide. The aim of this study was to identify oxa and ndm genes by multiplex-PCR and to determine the pattern of antibiotic resistance.
Material and Methods: This study was performed in a period of 8 months by collecting 25 bacterial plate isolates. Antibiotic susceptibility testing was performed on Müller Hinton agar medium by disk diffusion method. MPprimer software was used to design the primers. After data collection, the level of significance was assessed at P˂0.05 using SPSS 22.
Results: Except for gentamicin, cefpime, ciprofloxacin and tobramycin, there is a significant relationship between resistance pattern and sensitivity. All A. baumannii isolates were sensitive to ceftazidime. Also, 20% of the isolates were resistant to imipenem, which are considered carbapenem-resistant isolates of A. baumannii. Of the 25 isolates studied, 5 isolates (20%) had oxa58 gene, 5 isolates (20%) had oxa23 gene and three isolates (12%) had ndm gene.
Conclusion: The high prevalence of antibiotic resistance genes in A. baumannii isolates emphasizes the need for a systematic program in the control and treatment of this pathogen and shows necessity for more attention from health centers in prescribing appropriate antibiotics. Multiplex PCR method is a sensitive and reliable method for rapid detection of resistance genes in A. baumannii isolates.
Material and Methods: This study was performed in a period of 8 months by collecting 25 bacterial plate isolates. Antibiotic susceptibility testing was performed on Müller Hinton agar medium by disk diffusion method. MPprimer software was used to design the primers. After data collection, the level of significance was assessed at P˂0.05 using SPSS 22.
Results: Except for gentamicin, cefpime, ciprofloxacin and tobramycin, there is a significant relationship between resistance pattern and sensitivity. All A. baumannii isolates were sensitive to ceftazidime. Also, 20% of the isolates were resistant to imipenem, which are considered carbapenem-resistant isolates of A. baumannii. Of the 25 isolates studied, 5 isolates (20%) had oxa58 gene, 5 isolates (20%) had oxa23 gene and three isolates (12%) had ndm gene.
Conclusion: The high prevalence of antibiotic resistance genes in A. baumannii isolates emphasizes the need for a systematic program in the control and treatment of this pathogen and shows necessity for more attention from health centers in prescribing appropriate antibiotics. Multiplex PCR method is a sensitive and reliable method for rapid detection of resistance genes in A. baumannii isolates.
Type of Study: Research Article |
Subject:
Physiology
Received: 2024/05/19 | Accepted: 2024/05/12 | Published: 2024/05/12
Received: 2024/05/19 | Accepted: 2024/05/12 | Published: 2024/05/12
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