Aim and Background: The Following outbreak of the corona virus pandemic on December 12, 2019 and the declaration of a state of emergency by the World Health Organization, all researchers and researchers focused on dealing with this virus. opinion to scientists, one of the solutions to deal with the spread of the corona virus was to cut off the chain of transmission through disinfection and disinfection of high-traffic places such as airports, passenger terminals, subway stations with chemicals. Therefore, the World Health Organization presented the disinfection of high-traffic places and urban gathering points in its guidelines. The purpose of this research project is to study the disinfection power of chlorine dioxide solution in the concentrations provided by the manufacturer on spore-bearing and non-spore-bearing bacteria in order to introduce a safe and efficient disinfectant to be used against the corona virus.

Materials and Methods: In this study, which is descriptive, the disinfection power of chlorine dioxide solution on Escherichia coli and Klebsiella pneumoniae bacteria at concentrations of 1.100, 1.400, 1.800 and 1.1000 in 10 and 20 and 30 minutes were studied in three culture mediums: Blood agar, MHA and EMB. Also, the disinfecting power of the mentioned solution on Bacillus subtilis spore bacteria in concentrations of 1.400, 1.500, 1.600 and 1.700 for a period of 30 minutes and in tryptic broth medium was investigated. The results were analyzed by SPSS version 21 software.

Results: Based on the results, the chlorine dioxide solution in all the desired dilutions destroys Escherichia coli and Klebsiella pneumoniae bacteria in 20 minutes. Also, chlorine dioxide only at a dilution of 1.400 and a duration of 30 minutes caused the destruction of Bacillus subtilis spore bacterium.

Conclusion: In this study, vital information was provided in the field of effective disinfection strategy of chlorine dioxide against spore-forming and non-spore-forming bacteria with different dilutions, which can be used in different dimensions for disinfection of high-traffic urban areas.