Volume 14, Issue 56 (9-2024)
NCMBJ 2024, 14(56): 83-94 |
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Ojani S, Montazeri N, Masoud Mohammadi Zeydi M, Ghane M. Investigation of Antioxidant, Antibacterial, and Cytotoxic Effects of the Hydroalcoholic Extract of Seeds of Polylophium involucratum (Pall.) Boiss. on the Cell Line of Anaplastic Thyroid Cancer. NCMBJ 2024; 14 (56) :83-94
URL: http://ncmbjpiau.ir/article-1-1705-en.html
URL: http://ncmbjpiau.ir/article-1-1705-en.html
1. Department of Chemistry and Medicinal Chemistry, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran , montazer1350@gmail.com
Abstract: (188 Views)
Aim and Background: Anaplastic thyroid cancer is one of the most malignant and lethal tumors known among humans. The purpose of this project was to investigate the antioxidant, antibacterial, and cytotoxic effect of the hydroalcoholic extract of seeds of Polylophium involucratum (Pall.) Boiss. on the cancer cell line of anaplastic thyroid cancer.
Material and methods: To this end, the seeds of Polylophium involucratum (Pall.) Boiss. Were collected from the heights of Javaherdeh, Ramsar and extracted using a microwave-assisted extraction (MAE). Then phytochemical tests (identification of secondary metabolites, for example, terpenoids, coumarins, tannins, saponins etc., measurement of the total amount of phenolic and flavonoid compounds (TPC & TFC), respectively, by Folin-Ciocalteu reagent (FCR) and aluminum chloride colorimetry methods, also determination of antibacterial activity by disk diffusion method (DDM), and finally, evaluation of the effect cytotoxicity was investigated on 8305C cancer cell line using MTT assay.
Results: Phytochemical screening of hydroalcoholic extract of Polylophium involucratum (Pall.) Boiss. seeds confirmed the presence of flavonoids, terpenoids, coumarins, cardiac glycosides, tannins, phenols, quinones, and saponins. The total amount of phenolic compounds and flavonoid compounds were, respectively, 12.93 ± 2 and 7.58 ± 7 mg/mL. The percentage of free radical inhibition was 57.70 ± 0.5 and the IC50 value was 0.66 μg/mL.The diameter of zone of inhibition (ZOI) in Escherichia coli and Staphylococcus aureus, Bacillus cereus was observed as 25, 10, and 10 mm, respectively. Moreover, cytotoxicity was observed at a concentration of 30 μg/mL in 24 hours, and its IC50 value was equal to 50.12 ± 0.02 µg/mL.
Conclusion: Considering the secondary metabolites such as phenolic compounds and flavonoids present in the hydroalcoholic extract of Polylophium involucratum (Pall.) Boiss. seeds and its significant biological properties, it can be concluded that this plant has the potential to be used as a suitable drug candidate against cancer in the future.
Material and methods: To this end, the seeds of Polylophium involucratum (Pall.) Boiss. Were collected from the heights of Javaherdeh, Ramsar and extracted using a microwave-assisted extraction (MAE). Then phytochemical tests (identification of secondary metabolites, for example, terpenoids, coumarins, tannins, saponins etc., measurement of the total amount of phenolic and flavonoid compounds (TPC & TFC), respectively, by Folin-Ciocalteu reagent (FCR) and aluminum chloride colorimetry methods, also determination of antibacterial activity by disk diffusion method (DDM), and finally, evaluation of the effect cytotoxicity was investigated on 8305C cancer cell line using MTT assay.
Results: Phytochemical screening of hydroalcoholic extract of Polylophium involucratum (Pall.) Boiss. seeds confirmed the presence of flavonoids, terpenoids, coumarins, cardiac glycosides, tannins, phenols, quinones, and saponins. The total amount of phenolic compounds and flavonoid compounds were, respectively, 12.93 ± 2 and 7.58 ± 7 mg/mL. The percentage of free radical inhibition was 57.70 ± 0.5 and the IC50 value was 0.66 μg/mL.The diameter of zone of inhibition (ZOI) in Escherichia coli and Staphylococcus aureus, Bacillus cereus was observed as 25, 10, and 10 mm, respectively. Moreover, cytotoxicity was observed at a concentration of 30 μg/mL in 24 hours, and its IC50 value was equal to 50.12 ± 0.02 µg/mL.
Conclusion: Considering the secondary metabolites such as phenolic compounds and flavonoids present in the hydroalcoholic extract of Polylophium involucratum (Pall.) Boiss. seeds and its significant biological properties, it can be concluded that this plant has the potential to be used as a suitable drug candidate against cancer in the future.
Keywords: Cytotoxicity, 8305C Cell Line, Anaplastic thyroid cancer (ATC), Antioxidant activity, Antibacterial activity, Polylophium involucratum (Pall.) Boiss., Secondary metabolites.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2024/12/16 | Accepted: 2024/09/22 | Published: 2024/09/22
Received: 2024/12/16 | Accepted: 2024/09/22 | Published: 2024/09/22
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