Volume 15, Issue 58 (4-2025)
NCMBJ 2025, 15(58): 39-48 |
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Asadi A, Asadi N. Molecular study of antibiotic resistance genes in Escherichia coli strains from broiler chickens in Kerman by multiplex PCR technique. NCMBJ 2025; 15 (58) :39-48
URL: http://ncmbjpiau.ir/article-1-1739-en.html
URL: http://ncmbjpiau.ir/article-1-1739-en.html
Islamic Azad University, Shahr Babak Branch , dr.asadi44@yahoo.com
Abstract: (240 Views)
Aim and Background: Avian pathogenic E. coli is responsible for an extremely wide range of extraintestinal diseases in poultry, such as colibacillosis, cellulitis, coligranuloma, and yolk sac infection. The aim of this study is the molecular study of antibiotic-resistant resistance genes, such as bla, aac, aad, qnr, sul, tet, and cat, by the multiplex PCR method and the determination of antibiotic susceptibility.
Materials and Methods: One hundred and twelve strains of E. coli from colibacillosis cases and fifty-six strains from cellulitis cases were isolated from poultry slaughterhouses in Kerman province during eight months in 2021. The strains of E. coli were confirmed by biochemical methods, and then the PCR experiment and antibiotic susceptibility test were performed on the samples.
Results: The results of the studies showed that 65.85% of the samples for the tetA gene and 64.65% of those for the tetB gene were positive. Percentage of genes qnrS (47.3), qnrB (24.09), sulI (28.91), dhfrV (41.32), dhfrA (26.5), aac (3)-1 (25.66), aadA (32.89), floR (28.95), sulII (43.31), and qnrA (22.30) %. None of the samples were positive with regard to blaoxa, blaCTX, blasHV and blaTEM genes. In this study, it was specified that 50-85 percent of isolates of all strains were resistant to one or more antibiotics. The highest percentage of resistance to antibiotics was, respectively, to tetracycline (94.18%), sulfamethoxazole (61.24), chloramphenicol (45.96), and trimethoprim (46.36) %.
Conclusion: In this study. Recognition of resistance patterns and microorganisms to susceptibility to antibiotics plays an effective role in the correct and suitable selection of antibiotics and infection control.
Materials and Methods: One hundred and twelve strains of E. coli from colibacillosis cases and fifty-six strains from cellulitis cases were isolated from poultry slaughterhouses in Kerman province during eight months in 2021. The strains of E. coli were confirmed by biochemical methods, and then the PCR experiment and antibiotic susceptibility test were performed on the samples.
Results: The results of the studies showed that 65.85% of the samples for the tetA gene and 64.65% of those for the tetB gene were positive. Percentage of genes qnrS (47.3), qnrB (24.09), sulI (28.91), dhfrV (41.32), dhfrA (26.5), aac (3)-1 (25.66), aadA (32.89), floR (28.95), sulII (43.31), and qnrA (22.30) %. None of the samples were positive with regard to blaoxa, blaCTX, blasHV and blaTEM genes. In this study, it was specified that 50-85 percent of isolates of all strains were resistant to one or more antibiotics. The highest percentage of resistance to antibiotics was, respectively, to tetracycline (94.18%), sulfamethoxazole (61.24), chloramphenicol (45.96), and trimethoprim (46.36) %.
Conclusion: In this study. Recognition of resistance patterns and microorganisms to susceptibility to antibiotics plays an effective role in the correct and suitable selection of antibiotics and infection control.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2025/04/28 | Accepted: 2025/04/3 | Published: 2025/04/3
Received: 2025/04/28 | Accepted: 2025/04/3 | Published: 2025/04/3
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